Clustered Regularly Interspaced Short Palindromic repeats (CRISPR) are prokaryotic DNA segments with short repetitions of base sequences. Cas9 is the first nuclease discovered. CRISPR Cas9 System refers to a prokaryotic immune system during which the Cas9 proteins use the CRISPR spacers to recognize and cut the foreign genetic elements. According to the mechanism, scientists apply it to genome editing.
CRISPR Cas9 system is recently considered as the most remarkable breakthrough in genome editing technology. Compared with the existing TALEN and ZFN, CRISPR Cas9 system can provide more precise DNA modification through the RNA-directed Cas9 nucleases. Previous studies have indicated that the on-target gene knockout rate is higher than that of TALEN and ZFN, and the CRISPR Cas9 system is more efficient and economical. CRISPR/Cas9, the most promising gene editing tool, has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases.
Synbio Technologies offers the design, construction and validation of the CRISPR Cas9 gene targeting system through our patent pending synthetic biology platform in a price-competitive and time-effective approach. We offer a one-stop solution for CRISPR Cas9 projects to achieve high genome editing efficiency.
CRISPR Cas9 applications:
Advantages of CRISPR Cas9
- Generate knock-in or knock-out cell lines
- Simultaneously knockout multiple targets
- High gene knockout/knock-in efficiency
- Construction and usage of simplicity
CRISPR Cas9 Services
|CRISPR Cas9 sgRNA Design Center||CRISPR Cas9 sgRNA Library Design and Screening|
|CRISPR Cas9 sgRNA Panel||Mammalian Cell Line Gene Editing|
|Yeast Genome Editing|
How to order CRISPR Cas9 Services
If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.
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1.Shalem O, Sanjana N E, Hartenian E, et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 2014, 343(6166): 84-87.
2.Cong L, Ran F A, Cox D, et al. Multiplex genome engineering using CRISPR/Cas systems. Science, 2013, 339(6121): 819-823.
2.Hsu P D, Lander E S, Zhang F. Development and applications of CRISPR-Cas9 for genome engineering. Cell, 2014, 157(6): 1262-1278.
3.Yang L, Güell M, Niu D, et al. Genome-wide inactivation of porcine endogenous retroviruses (PERVs). Science, 2015, 350(6264): 1101-1104.
4.Liang P, Xu Y, Zhang X, et al. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & cell, 2015, 6: 363-372.