Lentiviral vectors have a high transfection efficiency on mammalian cells and can provide an efficient way to integrate foreign gene into host chromosome. As Cas9 protein has a longer ORF, conventional methods (electrotransfection or chemical reagents transfection) have a lower efficiency of inserting Cas9 gene into host cell. Hence, high-efficiency expression lentiviral vectors can be used to construct Cas9-expressing stable cell lines.
Our lentiviral packaging service provides lentiviral expression Cas9/Cas9 Nicknase and Cas9-expressing stable cell lines. It can also enhance the targeting efficiency of Cas9 and enable stable gene expression in host cells, so that we can make sure the CRISPR-Cas9 system has a higher efficiency of gene editing.
- Higher transfection efficiency: Lentiviral vector has a transfection efficiency of nearly 100% on hard-to-transfect cell lines (Such as neurons, primary cells, stem cells and tumor cells, etc.).
- Large cloning capacity: The longest gene fragment that can be inserted is 8k.
- Multigene editing: It can construct Cas9-expressing stable cell lines and enable simultaneously knock-out multiple targets in the same cell.
Cas9 lentiviral vector sequence
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