CRISPR Cas9 sgRNA Panel
Due to its simplicity and ease of customization, the CRISPR-Cas9 gene editing system is a popular tool in genetic screening. Recently, more and more literature report that CRISPR-Cas9 is more effective, reliable, and specific than RNAi in gene knockout experiments. Gene knockout screening utilizing CRISPR-Cas9 has been widely used for target and gene identification in many research areas, such as cancer development, drug resistance, immune response, bacterial toxin susceptibility, etc.
With our proprietary CRISPR-Cas9 sgRNA design platform, Synbio Technologies has designed 10 sgRNAs for each target gene, and constructed the CRISPR-Cas9 sgRNA Panel libraries. The delivery of these libraries includes plasmid, lentivirus, ready-to-use sgRNA, and more.
We provide integrated sgRNA for every gene in panels promoting cancer development and evolution, cell cycle pathway target screening, and cell pathway genetics screening research.
CRISPR Cas9 sgRNA Panel Advantages:
- Highly customizable sgRNA panel design for any variety of target genes
- Flexible delivery format, including plasmid, lentivirus, ready-to-use sgRNA, etc.
- Proprietary Syno® 3.0 DNA synthesis platform: fast and efficient sgRNA panel construction
CRISPR Cas9 sgRNA Panel screening workflow
Available methods for lentivirus CRISPR-Cas9 sgRNA library screening includes screening of resistance targets against the following: the anticancer drug Vemurafenib, the antiαtoxin of Clostridium botulinum, and anti 6-sulfur guanine. Pooled Panel library screening combined with NGS technology can accurately identify the target genes affected by the screening condition(s). Synbio Tech offers two of the most common types of library screening: positive screening and negative screening.
Application of virus library – Screening condition – Identify the result
Gene list is related to the screening pathway, sequence cells that survive
Application of virus library – Screening condition/no selection control – NGS
Gene list is partially related to the screening pathway, sequence surviving cells and compare to a control to screen for genes of interest
|Screen Type||Method of Use|
|Positive/Forward Screening||-Application of sgRNA Panel
-Screening condition: most cells dead or failed to transfect into plasmid or virus
-Selection of surviving cells
-NGS of the surviving cells
-Obtaining the gene list related to the screening condition
|Negative/Reverse Screening||-Application of sgRNA Panel
-Perform next generation sequencing on control sample(no selection):in parallel, apply library
-Screening condition: most cells survived
-NGS of the surviving cells
-Sequencing result comparison between two groups of cells
-Determine the sgRNA that disappeared due to the insertion of screening condition
Human protein kinase sgRNA Panel
Synbio Technologies has developed sgRNA Panels for human protein kinase genes, including genes which regulate cell pathway, cell cycle, and cell signal transduction. Due to the sequence homology of the catalytic domain, over 92% of human protein kinase belongs to a huge category of protein kinases, which includes 9 different protein kinase families.
|Protein Kinase Families||Protein Kinases|
|AGC Protein Kinase Family||PKA、PKC, PKG, etc.，please click here to check the whole list|
|CAMK Protein Kinase Family||Calcium dependent and calmodulin dependent protein kinase，please click here to check the whole list|
|CK1 Protein Kinase Family||Casein kinase 1, etc.，please click here to check the whole list|
|CMGC Protein Kinase Family||CDK、MAPK、GSK3, CLK, etc.， please click here to check the whole list|
|STE Protein Kinase Family||Yeast Sterile7/11/20 protein kinase homologous analogues, etc.，please click here to check the whole list|
|TK Protein Kinase Family||Tyrosine protein kinase, etc.，please click here to check the whole list|
|TKL Protein Kinase Family||Tyrosine like protein kinase, etc.，please click here to check the whole list|
|Atypical Protein Kinase Family||ADCK、Brd, etc.，please click here to check the whole list|
|Other Protein Kinase Family||NEK、Wnk, etc.，please click here to check the whole list|
How to order CRISPR Cas9
If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.
+1 609 228 5911