Site Directed Mutagenesis Services

Mutagenesis

If the specific site on your gene or vector is not what you want or needs to do a key contrast to carry out the functional study of genes or elements, you may need to do a point mutation on its specific site or multiple sites. Generally speaking, there are different mutation strategies for different research purposes, but it can be divided into two categories: Site-directed Mutagenesis and Random Mutagenesis. Site-directed Mutagenesis is a very useful tool for gene research, which can efficiently change the character and characterization of the target protein.

Generally we introduce site-directed mutagenesis through PCR method, including the insertion, deletion and point mutation. Site-directed Mutagenesis technology has been widely used in the study of protein functional site structure, enzyme activity optimization, DNA component function or component interaction, gene therapy and so on.

Advantages

  1. Short TAT: common sequence can be delivered to you within 5 working days;
  2. Vast operating region: if the distance between two point mutations is <40bp, we deal it as one
  3. High quality: Synbio Tech. has rich experience in solving kinds of repetitive sequences, including hairpin structure, high GC, poly structure and other complex sequences perfectly
  4. Deliverability: the record of performance that Synbio Tech. keeps is the delivery of a single DNA fragment up to 100Kbp

Service TAT and price

Cloned fragment length Amount of point mutation TAT (Business day) Price
<2kb 1 10 inquiry
2 10 inquiry
3 10 inquiry
2kb-3kb 1 13 inquiry
2 13 inquiry
3 13 inquiry
3kb-5kb 1 16 inquiry
2 16 inquiry
3 16 inquiry

Delivery form

  1. 1 tube of 4μg dry powder DNA
  2. 1 tube of glycerol bacteria or puncture bacteria containing the corresponding plasmids
  3. Original peak diagram of sequencing results,.ab1 format
  4. COA file, including QC enzyme digestion verification figure

How to order


Tel
+1 732-230-3003

Email
service@synbio-tech.com

Fax
+1 609 228 5911

Online Inquiry
online inquiry submission form