Baculovirus-Insect Cell Expression System

The Baculovirus-Insect Cell Expression System is one of the most popular eukaryotic expression systems for research and industry applications. There are several advantages of using the baculovirus-insect cell expression system, such as improved solubility, the ability to incorporate large DNA fragments, and comprehensive post-translational modifications.

With our experienced scientific team, Synbio Technologies has developed our own baculovirus-insect cell expression technology platform. The production scale of this platform is up to 500 L. Combined with our advanced protein purification facilities, we can deliver recombinant protein products with purity 95% and above. Protein expression levels and success rates can be significantly improved using our proprietary NG®Codon Optimization software.

Baculovirus-Insect Cell Expression System Advantages:

Proprietary NG®Codon Optimization Technology: significantly improve protein expression levels and success rate.
Multiple purification strategies: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc., providing diversified choice of protein purities.

Comprehensive protein identification services: SDS-PAGE, Western Blot, MALDI-TOF, HPLC, etc.

Service Specifications:


Steps Specifications Deliverables Timeline
Gene synthesis (optional) NG®Codon Optimization
Gene synthesis
Plasmid DNA 1-2 weeks*
Subcloning Subcloning the target gene into expression vector Constructs 1 week
Baculovirus preparation Bacmid DNA preparation and infecting insect cell 5ml,>107pfu/ml baculovirus 3-4 weeks
Pilot protein expression and purification Pilot protein expression and purification
Test the expression level
Expression report 1-2 weeks
Large scale protein expression and purification Scale-up expression and purification Recombinant protein
QC report
1-2 weeks

* The delivery time of this step bay vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.

How to order


call
Tel
+1 732-230-3003
email
Email
service@synbio-tech.com
fax
Fax
+1 609 228 5911
online-request
Online Inquiry
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