Mammalian Cell Expression System

The Mammalian Cell Expression System is an ideal system for functional protein expression. Biological activity of recombinant proteins produced by this system closely resembles that of natural proteins. Because of its unique advantages, mammalian cell expression systems are widely used in the biopharmaceutical industry.

Mammalian Cell Expression System is one of the most popular eukaryotic expression system for research and industry applications. There are several advantages of using baculovirus-insect cell expression system, such as improved solubility, ability to incorporate large DNA fragments, and comprehensive post-translational modifications.

With our experienced scientific team, Synbio Technologies has developed our own mammalian cell expression technology platform, capable of delivering recombinant proteins from the milligram level to the gram level. Combined with our advanced protein purification facilities, we can deliver recombinant protein products with purity 95% and above. Protein expression levels and success rates can be significantly improved using our proprietary NG® Codon Optimization software.

Mammalian Cell Expression System Advantages:

  • Proprietary NG®Codon Optimization Technology: significantly improve protein expression level and success rate.
  • Proprietary signal peptide library: customize signal peptide according to the sequence of target protein.
  • Multiple purification strategies: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc., providing diversified choice of protein purities.

Comprehensive protein identification services: SDS-PAGE, Western Blot, MALDI-TOF, HPLC, etc.

Service Specifications:


Steps Specifications Deliverables Timeline
Gene synthesis (optional) NG® Codon Optimization
Gene synthesis
Plasmid DNA 1-2 weeks*
Subcloning Subcloning the target gene into mammalian cell expression vector Constructs 1 week
Pilot protein expression and purification Pilot protein expression and purification
Test the expression level
Expression report 1-2 weeks
Large scale protein expression and purification Scale-up expression and purification Recombinant protein
QC report
1-2 weeks


* The delivery time of this step may vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.

How to order


call
Tel
+1 732-230-3003
email
Email
service@synbio-tech.com
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+1 609 228 5911
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