Synthetic DNA Libraries

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  • Generation of virtually any library type scanning, degenerate codon, triplet codon, modular etc.
  • Error-free gene synthesis – 100% sequence accuracy guaranteed
  • Libraries designed by your own or by our experts
  • Libraries available as Error-Free Clones, pooled clones, ds PCR fragments or as ss oligo mix
  • DNA clones provided in vectors or other forms as required
  • Sequence diversity verified and quality assured without any compromise

Applications of Synthetic DNA Libraries

Alanine Scan Library:

Research on amino acid residues critical to protein function, interaction and shape by replacing an individual amino acid, with alanine, at every position.
Alanine-Scan-Library

Site Saturation Library:

Substitute all 20 possible amino acids at any site to evaluate structure-function relationships and to generate improved protein variants.
Site-Saturation-Library

Random Substitution Library:

Substitute bases with triplet, random or degenerate ones to identify locations for specific analysis.
Random-Substitution-Library

Antibody Library:

Create a high diversity synthetic antibody library or improve existing antibody functionality such as specificity, immunogenicity, affinity, expression and/or aggregation.
Antibody-Library

Modular Substitution Library:

Build new genetic modules, regions or pathways from DNA parts including regulatory elements and/or coding sequences.

Modular-Substitution-Library

Deliverables for Synthetic DNA Libraries

Pooled ss Oligo Libraries Pooled ds PCR Libraries Pooled Cloning Libraries Error Free Separated Colonies Libraries
Length Up to 125nt/200nmol Up to 3kb Up to 109 transformants Up to 1M colonies
Fidelity Oligo mix with degenerated base(s) or triplet base(s) PCR fragment pool with variants Pool of variant sequences cloned into vector 100% sequence verified clones in a vector
Complexity high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes 30-80% GC, no repeats high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes
Custom Vector Available Available
Quality Assurance Amplification, Cloning and sequencing at least 16-96 clones or NGS to verify diversity Cloning and sequencing at least 16-96 clones or NGS to verify diversity Sequencing at least 16-96 clones or NGS to verify diversity 100% sequence verification using Next Generation and Sanger sequencing methods

Pricing and Delivery Times

Length (nt/kb) Complexity Analysis Diversity Analysis by Sequencing Oligo or PCR fragment Yield Pooled Cloning Plasmids/Culture Yield Error-free Separated Plasmids Yield Pooled ss Oligo Libraries Pooled DNA Library Priced Clone Library List Price Delivery Time*
0.02-0.125 100 nmol Inquire 10-15 Business Days
0.125-3.0 2 μg 20-100 μg/1-5ml culture 1-5 μg $0.15/bp plus degerated/triplet price $0.35/bp plus degerated/triplet price 10-15 Business Days
1.5-3.0 2 μg 20-100 μg/1-5ml culture 1-5 μg $0.20/bp plus degerated/triplet price $0.45/bp plus degerated/triplet price 10-15 Business Days
3.0-6.0 20-100 μg/1-5ml culture 1-5 μg $0.55/bp plus degerated/triplet price 20-40 Business Days
6.0-9.0

20-100 μg/1-5ml culture 1-5 μg $0.65/bp plus degerated/triplet price 30-50 Business Days
>9.0 20-100 μg/1-5ml culture 1-5 μg $0.95/bp plus degerated/triplet price Inquire

How to Order Synthetic DNA Libraries

If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.

online-quotation
Online Inquiry
email
service@synbio-tech.com
call
+1 732-230-3003
fax
+1 609 228 5911