- Generation of virtually any library type scanning, degenerate codon, triplet codon, modular etc.
- Error-free gene synthesis – 100% sequence accuracy guaranteed
- Libraries designed by your own or by our experts
- Libraries available as Error-Free Clones, pooled clones, ds PCR fragments or as ss oligo mix
- DNA clones provided in vectors or other forms as required
- Sequence diversity verified and quality assured without any compromise
Applications of Synthetic DNA Libraries
Alanine Scan Library:
Research on amino acid residues critical to protein function, interaction and shape by replacing an individual amino acid, with alanine, at every position.
Site Saturation Library：
Substitute all 20 possible amino acids at any site to evaluate structure-function relationships and to generate improved protein variants.
Random Substitution Library：
Substitute bases with triplet, random or degenerate ones to identify locations for specific analysis.
Create a high diversity synthetic antibody library or improve existing antibody functionality such as specificity, immunogenicity, affinity, expression and/or aggregation.
Modular Substitution Library：
Build new genetic modules, regions or pathways from DNA parts including regulatory elements and/or coding sequences.
Deliverables for Synthetic DNA Libraries
|Pooled ss Oligo Libraries||Pooled ds PCR Libraries||Pooled Cloning Libraries||Error Free Separated Colonies Libraries|
|Length||Up to 125nt/200nmol||Up to 3kb||Up to 109 transformants||Up to 1M colonies|
|Fidelity||Oligo mix with degenerated base(s) or triplet base(s)||PCR fragment pool with variants||Pool of variant sequences cloned into vector||100% sequence verified clones in a vector|
|Complexity||high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes||30-80% GC, no repeats||high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes||high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes|
|Quality Assurance||Amplification, Cloning and sequencing at least 16-96 clones or NGS to verify diversity||Cloning and sequencing at least 16-96 clones or NGS to verify diversity||Sequencing at least 16-96 clones or NGS to verify diversity||100% sequence verification using Next Generation and Sanger sequencing methods|
Pricing and Delivery Times
|Length (nt/kb)||Complexity Analysis||Diversity Analysis by Sequencing||Oligo or PCR fragment Yield||Pooled Cloning Plasmids/Culture Yield||Error-free Separated Plasmids Yield||Pooled ss Oligo Libraries||Pooled DNA Library Priced||Clone Library List Price||Delivery Time*|
|0.02-0.125||√||√||100 nmol||–||–||Inquire||–||–||10-15 Business Days|
|0.125-3.0||√||√||2 μg||20-100 μg/1-5ml culture||1-5 μg||–||$0.15/bp plus degerated/triplet price||$0.35/bp plus degerated/triplet price||10-15 Business Days|
|1.5-3.0||√||√||2 μg||20-100 μg/1-5ml culture||1-5 μg||–||$0.20/bp plus degerated/triplet price||$0.45/bp plus degerated/triplet price||10-15 Business Days|
|3.0-6.0||√||√||–||20-100 μg/1-5ml culture||1-5 μg||–||–||$0.55/bp plus degerated/triplet price||20-40 Business Days|
|–||20-100 μg/1-5ml culture||1-5 μg||–||–||$0.65/bp plus degerated/triplet price||30-50 Business Days|
|>9.0||√||√||–||20-100 μg/1-5ml culture||1-5 μg||–||–||$0.95/bp plus degerated/triplet price||Inquire|
How to Order Synthetic DNA Libraries
If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.
+1 609 228 5911