Design and Synthesis of Gene Circuits



One of the main goals of synthetic biology is to make biological systems rebuilding and re-tooling easier. Synthetic biologyis commonly referred asbiological engineering, with principles similar to how people assemble computers with various spare parts. Synthetic biologists hope to standardize core bioelements (such as enzymes, genetic circuits, metabolic pathways, etc.) and integrate these standardized ” bio-elements ” step by step to construct systems with specific functions and even synthesize new biological individuals.

The emergence of a new generation of gene synthesis technologies is represented by our Syno® 3.0 Synthesis platform. This platform is utilized in the construction of metabolic pathways or the synthesis of small genomes with high throughput and low-cost margins. With the development of sequencing and gene synthesis technologies, scientists areattempting to reprogram gene circuits and applications to change the direction of biological functions. Presently, synthetic biology has been widely applied in research fields including biology, medicine and new species synthesis.

  • In 2003, Jay Keasling and his research team were the first to reconstruct the biosynthetic pathway of artemisinic acid in E.coli. This accomplishment was the first functional verification of this metabolic pathway. Over the next 10 years, Jay Keasling and his team positively improved host selection and optimization, and industrialization.
  • Some important natural products, such as terpenoids, polyketides, non-ribosomal peptides, alkaloids etc. have been successfully synthesized through ectopic reconstruction of metabolic pathways.
  • In 2010, Craig Venter and his research team replaced mycoplasma cells in wild goats with genomic DNA from chemically synthesized Mycoplasma mycoides JCVI-syn1.0. In doing so, they successfully achieved self-duplication, which had the milestone significance in “artificial life”.

Research Strategy

design-and-synthesis-of-gene-circuits-01
  • Verify gene
    circuitelements
  • Design and
    construct gene circuits
  • Optimize expression
    level by Codon optimization for expression
  • Construction and assembly of in each pathway module
  • Transformation and synthesize of The optimal strains
  • Segregating validation and quality analysis of metabolites
  • Improvement and determination of metabolic pathways
  • Study and determine scaled production
  • Expand the application prospects of gene circuits

Service Advantages

  • One-Stop Solution
  • Synbio Technologies’ complete Syno® 1.0, 2.0, 3.0 DNA synthesis platforms provide one-stop service for all synthetic biology solutions.

  • Codon Optimization
  • Synbio Technologies has proprietary NGTM Codon Optimization Software, through which customers can optimize their sequences and design synthesis schemes for free.

  • Efficient Vector Construction
  • Synbio Technologies has a complete functional component library services. These services can successfully construct and verify the vectors with high throughput mechanisms. Using these mechanisms, we have successfully constructed vectors up to 100 kbp.

  • Professional Assembly Technology
  • Synbio Technologies’ self-developed synthetic assembly technology provides customized and cost-effective gene synthesis services capable of constructing sequences up to 150 kbp in length.

Reference
[1]Vincent J J Martin, Douglas J Pitera, Sydnor T Withers, Jack D Newman& Jay D Keasling.Engineering a mevalonate pathway inEscherichia coli forproduction of terpenoids. Nature Biotechnology 21, 796 – 802 (2003) .
[2]Neumann H, Neumann-Staubitz P. Synthetic biology approaches in drug discovery and pharmaceutical biotechnology[J]. Applied Microbiology and
Biotechnology, 2010, 87(1): 75-86.
[3]Gibson DG, Glass JI, Lartigue C, et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science, 2010, 329(5987): 52–56.