Metabolic Pathway Synthesis



Since the renaissance of synthetic biology in 2000, the research of high value-added natural product metabolic engineering combining with synthetic biology has become one of the most promising fields. In 2003, Jay Keasling’s team firstly reconstructed and accomplished the functional verification of the artemisinin biosynthetic pathway in E.coli. Over the next 10 years, Keasling’s team successfully streamlined the process of host selection, optimization, and industrialization. The synthesis and construction of metabolic pathways along with the necessary assembly techniques play a fundamental supporting role in this kind of research.

Research Strategy

metabolic-pathway-synthesis-09

  • Confirm metabolic
    synthesis related
    enzymes of
    intermediates
  • Design metabolic
    pathways at all
    levels
  • Optimize expression
    level with
    NGTM Codon Technology
  • Construction and
    assembly of
    modules on
    each pathway
  • Selected strains
    are transformed
    and synthesized
  • Separation validation
    and quality analysis
    of metabolites
  • Improvement and
    determination of
    metabolic pathways
  • Research to achieve
    scaled production
  • Expand the potential
    applications of
    metabolic pathways

Service Advantages

  • One-Stop Service
    Synbio Technologies has complete Syno® 1.0, 2.0 and 3.0 DNA Synthesis Platforms which provides one-stop synthetic biology solutions.
  • Codon Optimization
    Synbio Technologies has independent intellectual NGTM Codon optimization software, through which customers can optimize sequences and design synthesis schemes for free.
  • Efficient Vector Construction
    Synbio Technologies has a comprehensive functional component library, which can complete all construction and verification services with high throughput and has successfully constructed a vector up to 100 kbp.
  • Professional Assembly Technology
    Synbio Technologies’ self-developed synthetic assembly technology provides customizable and cost-effective gene synthesis and assembly services. This technology is capable of assembling single DNA sequences up to 150 kbp.