Syno®-Synthetic DNA Libraries

Synthetic DNA Libraries
Please contact our experts at support@synbio-tech.com to discuss your library design or submit your project idea electronically to receive a free quotation.
Features

  • Generation of virtually any library type scanning, degenerate codon, triplet codon, modular etc.
  • Error-free gene synthesis – 100% sequence accuracy guaranteed
  • Libraries designed by your own or by our experts
  • Libraries available as Error-Free Clones, pooled clones, ds PCR fragments or as ss oligo mix
  • DNA clones provided in vectors or other forms as required
  • Sequence diversity verified and quality assured without any compromise

Applications of Synthetic DNA Libraries

Alanine Scan Library:

Research on amino acid residues critical to protein function, interaction and shape by replacing an individual amino acid, with alanine, at every position.
Alanine-Scan-Library

Site Saturation Library:

Substitute all 20 possible amino acids at any site to evaluate structure-function relationships and to generate improved protein variants.
Site-Saturation-Library

Random Substitution Library:

Substitute bases with triplet, random or degenerate ones to identify locations for specific analysis.
Random-Substitution-Library

Antibody Library:

Create a high diversity synthetic antibody library or improve existing antibody functionality such as specificity, immunogenicity, affinity, expression and/or aggregation.
Antibody-Library

Modular Substitution Library:

Build new genetic modules, regions or pathways from DNA parts including regulatory elements and/or coding sequences.

Modular-Substitution-Library

 

Deliverables for Synthetic DNA Libraries

Pooled ss Oligo Libraries

Pooled ds PCR Libraries

Pooled Cloning Libraries

Error Free Separated Colonies Libraries

Length Up to 125nt/200nmol Up to 3kb Up to 109 transformants Up to 1M colonies
Fidelity Oligo mix with degenerated base(s) or triplet base(s) PCR fragment pool with variants Pool of variant sequences cloned into vector 100% sequence verified clones in a vector
Complexity high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes 30-80% GC, no repeats high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes
Custom Vector Available Available
Quality Assurance Amplification, Cloning and sequencing at least 16-96 clones or NGS to verify diversity Cloning and sequencing at least 16-96 clones or NGS to verify diversity Sequencing at least 16-96 clones or NGS to verify diversity 100% sequence verification using Next Generation and Sanger sequencing methods

 

Pricing and Delivery Times

Length (nt/kb)

Complexity Analysis

Diversity Analysis by
Sequencing

Oligo or PCR fragment Yield

Pooled Cloning Plasmids/Culture Yield

Error-free Separated Plasmids Yield

Pooled ss Oligo Libraries

Pooled DNA Library Priced

Clone Library List Price

Delivery Time*

0.02-0.125

100 nmol

Inquire

10-15 Business Days

0.125-3.0

2 μg

20-100 μg/1-5ml culture

1-5 μg

$0.15/bp plus degerated/triplet price

$0.35/bp plus degerated/triplet price

10-15 Business Days

1.5-3.0

2 μg

20-100 μg/1-5ml culture

1-5 μg

$0.20/bp plus degerated/triplet price

$0.45/bp plus degerated/triplet price

10-15 Business Days

3.0-6.0

20-100 μg/1-5ml culture

1-5 μg

$0.55/bp plus degerated/triplet price

20-40 Business Days

6.0-9.0

20-100 μg/1-5ml culture

1-5 μg

$0.65/bp plus degerated/triplet price

30-50 Business Days

>9.0

20-100 μg/1-5ml culture

1-5 μg

$0.95/bp plus degerated/triplet price

Inquire

How to order


call
Tel
+1 732-230-3003
email
Email
service@synbio-tech.com
fax
Fax
+1 609 228 5911
online-request
Online Inquiry
online inquiry submission form