De Novo Antibody Design and Production

Antibody drugs are among the most promising therapies and experienced explosive growth as new drugs have been approved for various diseases. Recent advances in antibody discovery technologies have paved way for development of a plethora of new antibody therapeutics.

In the past antibodies are discovered by well-established in vivo immunization procedures or in vitro antibody library screening approaches. However, the exacerbate cost and relatively high failure rate of the traditional path of drug discovery and development has prompted the urgent need for in silico approaches, especially structure-guided approach. The expansion of data repositories including protein structures, sequences and NGS has significantly increased the availability of large-scale data for antibody discovery. The availability of such data sets can provide a significant impact in streamlining antibody discovery process.

Synbio Technologies provides fast, flexible and customized one-stop solutions for antibody discovery. GenoAb platform includes hybridoma/monoclonal antibody sequencing, immune repertoire sequencing and phage display. SynoAb platform is a structure-based in silico antibody discovery method. The platform can screen early hits for antigens for wet-lab evaluation, followed by affinity maturations using controlled permutation libraries. The synergy from GenoAb and SynoAb will offer highly flexible supports and services for complicated antibody discovery.

Featured Advantages

1. Powerful drug-like antibody bio-computation
  • Cloud computing
  • Supercomputing
3. Facilitate the development of “biologically superior” antibody drugs
  • Antibodies humanization
  • Affinity maturation
  • Full human antibody library design
2. Proprietary software platforms
  • High-throughput antibody modeling
  • Effective antigen-antibody docking
4. Therapeutic antibodies have been licensed

Service Specifications

ServiceDetailsTurnaround TimeDeliverablesPrice
Syno® Ab Antibody Design and Production
  • CDR construction
  • Antibody modeling
  • Epitope docking
  • Screening optimization
2-3 Weeks
  • Antibody sequence report
  • Structure analysis of specific targets (antigens)
  • Molecular simulation and molecular dynamics ordering
  • Computational ordering of antibody library (human or specific species)
  • Leads with the affinity greater than 10-9M
  • Antibody library or the synthesized leads antibodies
Inquire

* Customers can provide the antigen name or gene ID directly; If you have antigen crystal structure, please contact us directly.

Service flow

antibody-design-and-production-1

service-workflow

Case Study

1. Extract potential epitopes based on the interface between target and ligand protein.

ligand-protein

2. Construct antibody library based on 3D structure.
3. Place antibodies based on surface complementary with target.

place-antibodies

4. Refine “hitting” antibody- target to increase its stability according to amino acids bias at different locations in antibody.
5. Select the best antibody hits according to evaluation, optimize DNA and then synthesize gene/library using Syno® 3.0 synthesis.

Syno3.0-synthesis

6. Final data

    • Protein A based capture approach

protein-a-based-capture

    • Anti-human Fc based capture approach

Anti-human-Fc-based-capture

Designed 1~100 antibody sequences for the antigen, finding hits with affinity greater than 10-10M

Affinity test results

Loading Sample IDkon(1/Ms)kdis(1/s)KD (M)Full R^2
55_569.28E+032.70E-072.91E-110.992429
57_583.76E+035.60E-071.49E-100.995068
Positive control7.29E+048.90E-071.22E-110.996965