Bioinformatics Analysis of Antibody Sequencing Data

Synbio Technologies can quickly and accurately sequence and analyze antibody sequences with our advanced GenoTM Ab Platform. Using this platform, we can perform high quality de novo sequencing of multi-species’ (including mice, rats, Armenian hamsters, camels, rabbits, equines, bovines, canines, birds, etc.) monoclonal antibodies. The Syno® Ab Platform also has the capabilities to analyze data that may come from various antibody libraries and hybridoma technologies, making this platform very applicable. Additionally, our experienced bioinformatics analysis team can also provide professional data mining and annotation.

Competitive Advantages

  • We use custom primer sets and special reagents developed in-house to ensure high success rates.
  • Our unique internal data mining tool can remove redundancies and extract information of target antibody sequence.
  • Diversified NGS analysis platform has the powerful ability for antibody gene analysis, resulting in a high quality output.
  • Complete bioinformatics analysis removes redundant sequence data, and deeply integrates the high-value information.

Service Specifications

ServiceDescriptionTurnaround TimeDeliverablesPrice
Antibody Sequencing Bioinformatics AnalysisSequencing data mining, Antibody information analysis, etc.7 – 10 Business DaysAnalysis ReportsQuote

Standard Antibody Sequencing Service Process

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Case Study

Synbio Technologies’ immune repertoire sequencing and bioinformatics analysis:

  1. Library Construction
    Synbio Technologies performed 5’ RACE reverse transcription of the extracted RNA, amplified, and constructed the library.
  2. Library Quality Control
    The Qubit 2.0 Fluorometer was used to perform preliminary quantification after the library construction. After dilution, insert size detection was performed on the library based on the Agilent 2100 bioanalyzer. Only when meeting expected requirements, the effective concentration of the library (>2nM) was accurately quantified by qPCR to ensure the quality of the library.
  3. Library Sequencing
    Different libraries were pooled and sequenced by Illumina Miseq/HiSeq according to the effective concentration and the required amount of target data. Four fluorescent labeled dNTPs, DNA polymerase, and adaptors were added to the flow cell for the amplification. When each sequence cluster extended and generated the complementary chain, the corresponding fluorescent signals were released and captured after each new dNTP was added. The optical signals were then transformed into sequencing peaks by professional software to obtain the sequence information of the fragments.
  4. Data Analysis
    Quality assessment of sequencing data.
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Per base sequence quality
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Per Sequence Quality Scores
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Per Base Sequence Content
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Per Base GC Content
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Per Sequence GC Content
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Per Base N Content
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Sequence Length Distributionreads
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Duplicate Sequences