Category: DNA synthesis

Synthetic DNA Variant Library Synthesis Service

Synthetic DNA library synthesis is widely applied mechanism within various fields of research such as: discovery biology, protein engineering, structural biology, and gene expression. All of these fields of scientific research have revolutionized the world we live in today, through various means. One of the more popular discoveries in the genetics research community was the construction of the first synthetic DNA library. A synthetic DNA library is a pool of DNA fragm ents which is stored within a host micro-organism via molecular cloning. The resulting synthetic DNA library encodes variants of a common design and structure. Each fragment is stored in a separate host organism, such as bacterium. This offers a palpable advantage due to bacteria’s ability to quickly and accurately replicate the DNA to produce detectable quantities. This process allows us to have quick access to many different sequences of interest with ease and efficiency.

Compared with traditional methods for DNA synthesis

The traditional method of “design-build-test” routine to reach a genetic design to improve synthetic biology has been proven to be slow, poor, and unreliable. Due to this reason, an alternative method of generating a DNA libraries was discovered and utilized within the research community with higher frequency. With the Synthetic DNA library synthesis service, high performing genetic solutions will be worked out with ease and efficiency. The DNA library synthesis pool can achieve gene synthesis and cloning with a much faster, smarter, and more reliable approach. This allows for a much more accurate and efficient DNA library to be synthesized with the use of DNA synthesis. With a more accurate and efficient DNA library, various topics within genetics research will better understood and more effectively studied.

Synthetic DNA library synthesis makes biological engineering much more efficient

With DNA library synthesis based methods, highly complex sequences within synthetic biology can be engineered rapidly with high accuracy. It is a novel approach which allows for the customer specific DNA sequence to be more reliably synthesized and obtained with ease and higher accuracy. This also enables new biological systems and products to move toward potential revolutionary discoveries.

Custom Synthetic DNA library synthesis services

DNA synthesis allows us to synthesize gene libraries of any size and complexity, specific to the customer’s specifications. DNA library synthesis relies upon DNA synthesis, a method mastered by Synbio Technologies through our three phase Syno® Platform. This platform allows us to generate the customer specified DNA sequence of interest, up to and including 150 Kb in length, with one hundred percent accuracy. DNA synthesis is the technology that provides the foundation to meet the needs of the DNA library construction and maintenance. This strong foundation is important because DNA synthesis is the first step for generating DNA libraries. With the proper construction of DNA libraries researchers aim to identify novel genes and how each gene relates to various protein functions and structures.

DNA Synthesis Related Services

  • Codon Optimization
  • gene synthesis
  • Small Genome Synthesis
  • Pathway Synthesis

Progress in DNA Synthesis Steps

Within the last few decades, advancements in methods of DNA synthesis, as well as synthesizing, DNA sequences have improved our capabilities of understanding genomics which has led to improved capability of engineering biological systems. The chemically synthesized DNA oligonucleotides and their assembly constructs contain encoded genes, synthons, circuits, metabolic pathways and even whole genomes. These assemblies have the ability to design, build, and test various research topics within molecular technology and synthetic biology. At Synbio Technologies we pride ourselves in our ability to successfully synthesize the requested DNA sequence specific to each customer. We have the ability, through our Syno Gene Synthesis Platform, to synthesize sequences up to and including 200kb in length with guaranteed one hundred percent accuracy.

DNA synthesis from oligonucleotides

As a polymer, consisting of four different phosphoramidite monomers, DNA synthesis follows a form of hierarchical polymer synthesis. Within the more traditional method of synthesizing DNA, nucleotide monomers make a connection between each other in order to construct individual oligonucleotide. On the other hand, in a more modern method of conducting gene synthesis, for assembling a synthetic double-stranded DNA, namely synthon, requires that adjacent oligonucleotides should be equipped with overlapping sequences. Once the sequences are long enough, the sequences-verified synthons are assembled to produce synthetic DNA constructs. The synthetic DNA constructs can then be commonly found within metabolic pathways or whole genomes.

Traditional column-based oligonucleotide as a source of DNA synthesis has a limitation to cost reduction. The cost of that ties to the consumption of reagent use, the scale of synthesis, the length of the oligonucleotides and the technical supplier.

DNA synthesis from high-fidelity microchips

The demand of cheaper resources necessary for DNA synthesis has resulted in an interest in a more recently developed method of array-synthesized DNA. Within the original method of DNA synthesis, the oligonucleotides were attached to a microchip surface. This attachment was accomplished via applying a modified phosphoramidite synthesis steps. This promising route has the capability to generate up to a million diverse oligonucleotides for constructing DNA on an individual chip.

Admittedly, oligonucleotides from DNA microchips can minimize the cost of DNA synthesis by a significant magnitude. High-fidelity microchips are able to be optimized through controlling depurination during the process of synthesis.

Synbio Technologies has in placed Syno® 3.0 synthetic platform, which is a next generation chip based DNA synthetic technology. Tens of thousands of or even one hundred thousand primers for one-time synthesis can be achieved on a semiconductor chip. Synbio Technologies is competent to provide lower-cost methods to produce high-quality synthetic DNA to meet all customers’ request.

DNA Synthesis Related Services

  • Codon Optimization
  • gene synthesis
  • Small Genome Synthesis
  • Pathway Synthesis

DNA Synthesis Definition and Methods

DNA synthesis definition

DNA synthesis is defined as the process by which copies of nucleic acids are strung together to form a longer DNA sequence within a laboratory setting.

DNA synthesis processes

DNA oligomers are the foundation of the DNA synthesis process. The essential feature of DNA synthesis is that no naturally isolated DNA is used. This step deviates from the traditional method of studying genetics, by which a preexisting genetic sequence was extracted from an organism to be amplified and later studied. With gene synthesis we, at Synbio Technologies, are capable of generating de novo genetic sequences that may not currently be present anywhere within nature. Although clonal plasmid-based intermediates might exist during the assembly of a target DNA, every base originates as a phosphoramidite molecule at the beginning of DNA synthesis. Today, all DNA synthesis methods begin with solid-phase phosphoramidite chemistry to construct single-stranded DNA molecules ranging in size between 10 and 100 base pairs in length. These single stranded molecules are then enzymatically assembled into larger molecules. This process is then repeated until the required sequence length is generated. This process has been perfected by Synbio Technologies through our three phase Syno Platform. This platform offers the ability to generate sequences up to and including 150 Kb in length with one hundred percent accuracy, guaranteed to be identical to the sequence requested by the customer.

DNA synthesis assembly in yeast

A commonly used method of DNA fragment assembly is through the use of a yeast intermediate. The yeast, better known as Saccharomyces cerevisiae, can synthesize single-stranded DNA with a minimum length of 38 overlapping base pairs and a linear double-stranded vector in one transformation event. In this way, oligonucleotides can synthesize DNA for a wide range of lengths, ranging from as few as 20bps in length to as large as 200bps in length. DNA synthesis assembly in yeast is a useful method for building synthetic DNA molecules that are relatively large in length. Since yeast have a powerful capacity to synthesize and recombine DNA fragments, yeast has become a model eukaryote for studying numerous cellular processes. In addition to this advantage, homologous recombination in yeast can be used to build DNA fragments from overlapping constituent parts.

TopDown one-step DNA synthesis method

TopDown DNA synthesis is a simple and cost-effective method to assemble a long genetic sequences up to and including 150 Kb in length. The TopDown DNA synthesis method can be distinguished from conventional gene synthesis methods by two key features. First, the melting temperature of the outer primers is designed to be ~8°C lower than that of the assembly oligonucleotides. Secondly, there are different annealing temperatures that are utilized to selectively control the efficiencies of the oligonucleotide assembly and full-length template amplification. The TopDown DNA synthesis method is an effective way to remove the interference between PCR assembly and amplification. This interference between PCR assembly and amplification have been linked to the development of certain malfunctions within the DNA synthesis process. This demonstrates that TopDown DNA synthesis is a method which can optimize DNA synthesis under certain condition.

Synbio Technologies is a DNA technology company whose main focus is DNA synthesis. This is seen in our high quality products generated by Synbio Technologies’ proprietary Syno® DNA synthesis platform which can synthesize sequences up to and including 150 Kb in length. This length is characteristic of DNA sequences which make up genes, genomes, and various biological pathways. In addition to this, the professional Syno® Codon software can significantly optimize the original sequences from customers, and provide synthesized DNA sequences with one hundred percent accuracy within 5 days. It is these factors that have led Synbio Technologies to become one of the premier companies within the gene synthesis industry.

DNA Synthesis Related Services

  • Codon Optimization
  • Vector Construction
  • Small Genome Synthesis
  • Pathway Synthesis

Synbio Technologies’ DNA Synthesis Service

DNA synthesis is a method in synthetic biology for the creation of deoxyribonucleic acid biopolymers in a sequence-specific manner. DNA synthesis can be further classified into oligonucleotide synthesis and gene synthesis. Currently, oligonucleotides are manufactured almost exclusively using automated solid-phase methods.In this way, we can apply efficient and accurate DNA synthesis services. Oligonucleotide synthesis produces short, single-stranded nucleic acid polymers usually made up of 18 to 25 nucleotides, which are usually used as probes for detecting specific DNA/RNA sequences by recognizing and binding to a complementary segment of DNA/RNA. Oligonucleotides are widely used as DNA/RNA structure probes, DNA microarray probes, and gel electrophoresis references, and are usually single strand fragments limited to 100 nucleotides. On the other hand, gene synthesis is able to create double-stranded DNA ranging from 50 bp to 12,000 bp in length. Solid-phase based gene synthesis opens up new avenues for completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size.

Synbio Tech DNA Synthesis Service

Taking advantage of industrial-leading processing technology, Synbio Technologies’ Syno® 1.0 platform provides a full selection of conventional primers, ultra-high purity primers, degenerate primers, aptamers, and fluorescent probes for world-class scientific research and some industrial customers.Synbio Technologies can also manufacture large scale primer synthesis using multi-well plates to deliver up to 10,000 primers for customized orders.

Our Syno® 2.0 DNA synthesis platform is the most versatile platform empowered by patented Syno® DNA Assembly technologies. Syno® 2.0 platform can cope with any type of gene and genome ranging from 100 base pairs to 1 million base pairs that contain all types of complex sequences. Standard complexity order can be completed within 5 business days with 100% sequence fidelity. NGTM software is developed at Synbio Technologies to boost the expression of synthetic genes in all commonly used expression systems.

Next Generation DNA Synthesis Company

As a leading DNA synthesis company, Synbio Technologies offers a comprehensive package of synthetic genomics services, from simple oligonucleotides and DNA fragments to large and complex genome clusters. Our cutting edge Syno® 3.0 high-throughput platform is the world’s most advanced next-generation DNA synthesis platform, able to provide affordable synthetic DNA at unprecedented scale.

At Synbio Technologies, our DNA synthesis capacity is empowered by several patented technologies. Industry-Leading NGTM Codon can help customers redesign and enhance the efficiency of the genes to achieve superior gene expression. Syno® Assembly is truly one-of-a-kind technology, working in tandem with our Syno® 2.0 platform to ensure the delivery of affordable, error-free sequences.

In addition, Synbio Technologies is building up its first integrated Syno GPS (Genotype, Phenotype and Synotype) system, aiming to streamline translation or reverse translation between “Genotype” and “Phenotype” through our proprietary “Synotype” platform. Our company’s scientific capabilities encompass areas such as molecular biology and bio-pharmaceutical research, precision medicine, biotechnology, molecular breeding, and biofuel production.

Synbio Technologies, a DNA synthesis company devote to next generation DNA synthesis, provides a variety of synthetic genomic options to advance your research, including primer synthesis, gene synthesis, metabolic pathway synthesis, gene cluster synthesis, CRISPR-Cas9 service, Sanger sequencing, and NGS services.

DNA Synthesis Application

With the growth of DNA synthesis techniques, especially the focus on automation and automatic technologies, we can more easily, rapidly, and efficiently synthesize DNA fragments. The applications of synthetic DNA encompass a broad spectrum ranging from genetic circuits and metabolic pathways to synthetic genomes. DNA synthesis is an essential technique in molecular biology and plays a vital role in various disciplines, such as genetic engineering, and clinical diagnosis/treatment.

DNA Synthesis in Molecular Biology

According to the clarified nucleotide sequence and amino acid sequence, we can synthesis the polypeptide and protein gene which can be applied in actual production and research. So far, various genes have been synthetic and expressed successfully in eukaryotic system, such as human growth hormone, interferon, insulin, interleukin etc.

DNA Synthesis Applications in Medicine

As our technology has become more powerful, our understanding of many diseases has become more sophisticated, from the organ and tissue level to the molecular and genetic level. As a result, new discoveries have allowed us to tackle genetic diseases in ways that we could not previously. For example, fluorescent probes can detect potentially harmful deletions in certain genes, proving to be a valuable tool for clinical diagnosis.

DNA Synthesis Application in Synthetic Biology

A valuable domain of synthetic biology is metabolic engineering. By engineering parallel metabolic systems that interface with natural cellular metabolic machinery, researchers can program cells for practical applications including the synthesis of cost effective chemicals or drugs. In one demonstration by Keasling and colleagues, the construction of a biosynthetic artemisinin pathway in yeast enabled the microbial production of this antimalarial compound at one-tenth of the cost of conventional production method of harvesting from the rare Artemisia annua plant.

Synbio Technologies concentrates primarily on DNA synthesis technologies. We have established our proprietary Syno® gene synthetic platform which can provide a variety of services including oligonucleotide synthesis, fluorescent probe labeling, and synthesize gene/genome, pathway and DNA library. We can also provide materials and supplies for clients in various disciplines, such as biological medicine, molecular diagnosis, industrial enzyme, bio-fuel, modern agriculture, environmental protection and surveillance and DNA storage.

Gene Synthesis Services

At Synbio Technologies we offer a unique approach to gene synthesis services through our revolutionary Syno Gene Synthesis Platform. This three phase platform allows us to generate any customer specified genetic sequence in text format, up to and including 150 Kb in length. The requested sequences can vary greatly in both length and structure. For example, a requested sequence can contain a gene with a deleterious mutation in order to induce a debilitating effect, rendering the gene inactive in order to study the phenotypic effects. Or the requested sequence can be one not previously existing within nature, in order to study the effects of evolution or antibiotic resistance. These examples are made possible through Synbio Technology’s various gene synthesis services. First, our Syno® 2.0 Gene Synthesis plays a large role in small gene/short sequence synthesis. The Syno® 2.0 Gene Synthesis platform may be meant for short sequence synthesis, but it still has the capabilities and confidence of generating sequences with extreme accuracy of up to and including 150 Kb in length. Second, our Syno® 3.0 Next Generation Gene Synthesis Platform is a groundbreaking piece of technology that allows us to synthesize sequences that span near the length of small genomes. This gene synthesis services allows us to generate sequences up to an including 150 Kb in length, which was previously thought to be impossible to successfully and accurately engineer in a laboratory setting. With these two gene synthesis services, along with a wide range of other biological technology services, Synbio Technology is ready to supply our customers with the highest quality product within an efficient timeframe. This confidence starts with one of our most successfully gene synthesis services, the Syno® 2.0 Platform.

The Syno® 2.0 Gene Synthesis Platform is one of the corner stones of our gene synthesis services. This platform has various specializations in gene synthesis such as: pathway synthesis, small genome synthesis, vector construction, and codon optimization technology. With this platform, we are able to successfully and accurately synthesize the customer specified sequence of interest with one hundred percent accuracy. This is accuracy and confidence in sequence validity comes from the multiple occasions in which we use Sanger sequencing to verify the sequence being generated. In addition to the high quality sequence synthesis, we offer cloning of each prepared sequence to ensure the customer is provided with the adequate amount of product. Before each sequence is amplified, it is verified again with Sanger sequencing to verify the accuracy of the sequence. In addition to the specializations Syno® 2.0 offers, this platform also offers the ability to generate various structures of DNA commonly utilized in various fields of genetics research. Some of these structures include: hairpin structures, repeated sequences, high GC content regions, high AT content regions, or multiple consecutive nucleotide sequences. All of these structures offer a unique approach to studying various topics within the field of genetics. With the Syno® 2.0 Platform, and our other gene synthesis services, Synbio Technologies is ready to offer our customers a unique approach to conducting genetic research.

The Syno® 3.0 Next Generation Gene Synthesis Platform differs slightly from the Syno® 2.0 Platform, and our other gene synthesis services, but the output is still guaranteed to be one hundred percent accurate with a low price and fast turnaround time. The Syno® 3.0 Platform specializes in ChipBased DNA synthesis, CRISPR-Cas9 Libraries, and digital information storage. In other words, the Syno® 3.0 Platform is one of our gene synthesis services that offer a large amount of generated sequences to be synthesized. The chip-based gene synthesis approach allows us to generate thousands upon thousands of sequences all within a single chip. This allows for rapid production of sequences with high accuracy when multiple chips are used. The generated sequences are then, of course, verified with Sanger sequencing to guarantee one hundred percent accuracy. This method is used to generate large genes, up to and including 150 Kb in length with one hundred percent accuracy. In addition to this, the Syno® 3.0 platform gene synthesis services that Synbio Technologies offers, is the use of and experience with CRISPR-Cas9. Ever since the discovery and first successful utilization of CRISPR-Cas9, in 1993, it has been used time and time again in order to conduct genome edits. CRISPR-Cas9 has become one of the most popular and effective methods in order to conduct genome edits. This gene synthesis service offers CRISPR-Cas9 based gene knockout-out, gene knock-in, transcription activation, and transcription inhibition. All of these applications are methods of altering the expression level of a particular sequence/gene of interest. This alteration of expression will then be applied to various types of genetics research.

All of the gene synthesis services, as much as they vary in application, offered by Synbio Technologies have been tested and proven to be one hundred percent effective. This comes in the form of our guaranteed sequence validation, through Sanger sequencing. This accuracy and validity have given Synbio Technologies a reputation as one of the premier companies in the gene synthesis industries. This reputation relies heavily on our Syno® Gene Synthesis Platform. With this gene synthesis service platform, we are able to synthesize the sequence/gene of interest with extreme accuracy in a cost effective manner. This cost effective manner, on top of the efficient turnaround time, we at Synbio Technologies are ready to supply our customers with the highest quality product for the lowest prices.

Gene Synthesis Related Services

  • Codon Optimization
  • Vector Construction
  • Small Genome Synthesis
  • Pathway Synthesis

Gene Synthesis Definition

The traditional method of studying a gene’s function is to extract the gene of interest, amplify it and put the resulting sequence through various molecular assessments. These assessments can vary from generating a frameshift mutation within the sequence, to upregulating the expression of the sequence in order to observe the resulting phenotypes the mutated sequence has induced on the organism. All of these methods are particularly important within the field of genetics research, but the methods of extracting these sequences to then mutate and study is becoming laborious and cumbersome. One, relatively recent and groundbreaking method, gene synthesis , has taken this traditional method of studying genetics and introduced some interest alterations. Gene synthesis is defined as the synthetic biology method used to engineer artificial genes within a laboratory setting. This method has allowed us, at Synbio Technologies, to go from a sequence in text format to physical copy of the genetic sequence itself with ease and extreme accuracy. Ever since gene synthesis was successfully conducted in 1972, by Hard Gobind Khorana and associates, this technology has been growing not only in popularity but in effectiveness and efficiency. One of the main reasons this technology has increased in popularity is that it allows for the creation of long, up to 150 Kb, sequences to be synthesized with extremely high accuracy. With the capability of generating sequences this large, gene synthesis gives a powerful approach to studying various topics such as complex pathways and generating synthetic DNA libraries. This high accuracy and relatively long length have promoted gene synthesis to be applied to various fields of genetics and biological research topics.

The fields of biological research that gene synthesis has been applied to vary from targeted mutagenesis in order to study a gene’s function to agriculture through increasing crop production. One of the main advantages that gene synthesis offers it the ability for a customer to request a specific sequence of DNA in text format up to 150 Kb in length. This text format is then converted into a physical copy with relative ease and high accuracy. The resulting sequence is then amplified an adequate amount of times in order to comply with the customer’s request. The accuracy allows the customer to generate a mutation anywhere within a gene of interest specific to their research topics. Not only can the mutation be generated anywhere, but any type of mutation can be used: frameshift, deamination, deletion, insertion, or nonsense mutation. In addition to this, gene synthesis can also be used to upregulate a gene of interest in order to cause an overexpression of the resulting phenotype. All of these mutations can be requested and inserted or applied the sequence of interest in order to obtain a better understanding of different types of genetics research such as evolution, or antibiotic resistance. In addition to these applications, gene synthesis has also been applied to agriculture through use of genetic engineering. Gene synthesis is the basis of genetic engineering, allowing for the creation of a resistance gene to a particular bacteria for example. The advantage that gene synthesis has to offer here is the ability to generate mutations within the synthesized gene to keep up with the constantly evolving bacteria. Gene synthesis through genetic engineering, have helped grow the agriculture industry, as well as develop a better understanding on how to improve crop survivability in varying climates as well as exposure to certain harmful bacteria. These applications have large impacts on not only the field of genetics, but the world. This is the main reason as to why Synbio Technologies is proud to be one of the premier companies within the gene synthesis industry.

At Synbio Technologies, we try to keep our gene synthesis process as simple as its definition. Gene synthesis is carried out with ease and extremely high accuracy through our Syno® Gene Synthesis Platform. This three phase platform allows us to generate the physical genetic sequence of the customer specified request. This generated sequences is guaranteed to be one hundred percent identical to the customer requested sequence. The guarantee is backed by Sanger sequencing at multiple phases of the three platforms. The resulting gene synthesis product will then be shipped to the customer specific location within an efficient timeframe. This entire process allows Synbio Technologies to offer the highest quality product, with an efficient turnaround time. These two aspects, combined with competitive prices and respectable reputation, it is clear that Synbio Technologies is one of the premier companies within the gene synthesis industry.

Gene Synthesis Related Services

  • Codon Optimization
  • Vector Construction
  • Small Genome Synthesis
  • Pathway Synthesis