Category: Gene Synthesis

Introduction of A New DNA Synthesis Method

Gene synthesis is the main content of current synthetic biology. Through gene synthesis, genes that do not exist in nature can be obtained, which provide a whole new direction for humans to transform organisms. In the foreseeable future, gene synthesis will play an important role in the field of life sciences. However, the current speed of gene synthesis still cannot fully meet the needs of biological development. A few days ago, a new document published by “Nature Biotechnology” pointed out that a new gene synthesis technology actually shortened the synthesis time of new genes to only one day!

Traditional gene synthesis is mostly used for the creation of antibody genes, primarily using terminal deoxynucleotide transferase (TdT), which can add new nucleotides to the oligonucleotide chain without following the DNA template chain. Thereby, new variants of millions of antibody genes have been written, and the immune system can use these variants to target invaders. But, natural TdT can only randomly add new deoxynucleotides, and researchers currently cannot accurately control the synthesis process.

gene-synthesis

DNA sequence synthesis process under the action of TdT

The researchers of this article designed the TdT-dNTP conjugate, which can be actively covalently linked to the 3’end when incorporated into the primer. This prevents the binding of other TdT-dNTP conjugates to the primer. During further extension, the linker can be cleaved, the 3’end of the primer is deprotected, and a single nucleotide is added to the extension. This makes the “termination” process reversible from now on, and researchers can turn on or end the role of TdT as required.

The researchers have shown that the active TdT-dNTP conjugate can connect the nucleoside triphosphate to various points on the surface of the polymerase through two different linkers. They found that the TdT-dNTP conjugate can achieve DNA in 10-20s within one single nucleotide extension of the molecule. So, this cycle of extension and deprotection reaction can quickly and accurately write a defined sequence, and it takes only one day to synthesize a complete gene.

dna-synthesis

TdT-dNTP achieves single nucleotide extension of DNA molecules within 10-20s

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References

Palluk S , Arlow D H , Rond T D , et al. De novo DNA synthesis using polymerase-nucleotide conjugates[J]. Nature Biotechnology, 2018, 36(7).

Gene synthesis in synthetic biology

Gene synthesis is a kind of technique that has been used to synthesize double-stranded DNA molecules in vitro by artificial ways. It is different from the single-stranded oligonucleotide synthesis in term of the sequence length. The longest synthesized oligonucleotide fragment is just up to 100nt. However, double-stranded DNA molecules, as products of the gene synthesis, can reach 50bp-12 kbp in length. Besides, compared with the approach of gaining genes from existing organisms, gene synthesis do not need templates, so it is not restricted by gene donors.

The first artificially synthesized gene appeared in the 1960s. As a main subject in current synthetic biology, gene synthesis sets a new direction for human beings to change organisms in gene level. It is predictable that in the near future gene synthesis will play a significant role in life science. Moreover, its advantages in new energy, new materials, artificial organisms, DNA vaccines, and biomedicine, have initially been noted by scientists.

Synbio Technologies provides our customers comprehensive DNA solutions that can satisfy all their DNA manufacturing needs. Our Syno® DNA synthesis platforms provide gene and fragment synthesis, high-throughput DNA synthesis, pathways synthesis, genome assembly, etc. Our proprietary algorithm based on DNA sequence complexity index (CI) system can precisely facilitate the success of complex sequences, for example: repeated sequences, high/low GC content, hairpin and poly structure, ultra-long sequences (up to 150 kb), etc. We have developed the most complete platforms of oligo synthesis, gene synthesis (fragment and clonal genes) and next-generation high-throughput gene synthesis to meet our customers’ needs in quality, speed, budget, and applications.

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High-throughput gene synthesis

Synthetic biology research began with the redesign, reconstruction, and optimization of existing life systems, so that the transformed biological system can meet the purposeful engineering applications of human beings. It has now changed from a single gene research model to integrated modules and loops, based on multi-gene and systemic research on pathways (even genome-wide).

In July of 2016, American scientists proposed the Human Genome Writing Program (HGP-Write), with the goal of synthesizing a complete human within 10 years . The haploid genome consists of approximately 3 billion base pairs. The rapid development of synthetic biology, especially the start of the HGP-Write project, has made the demand for synthetic genes exponentially increase. A new generation of large-scale, low-cost, high-throughput gene synthesis and assembly technologies become a driving force in synthetic biology.

Synbio Technologies Synotype platform includes complete Syno® 1.0 – Syno® 3.0 DNA synthesis platforms. These three platforms have enabled us to form a complete DNA synthesis biological technology system, leading us in becoming an industry leader. As a reliable supplier of the new generation high-throughput chip DNA synthesis, we can parallelly synthesize tens to hundreds of thousands of primers onto a single semiconductor chip. Our innovative design and synthesis process and quality management ensure to providet the highest quality DNA fragments (including primers, elements, and genes), whole-gene synthesis, pathway synthesis, gene assembly, nucleotide pools, and various mutation library synthesis. Our genetic products and customized services cover the synthesis, construction, and shuttle of any DNA fragment into any vector system.

  • Our Syno® 1.0 – Syno® 3.0 complete DNA synthesis technology platforms are designed to meet our customers’ individual needs in terms of throughput, delivery cycle, price, difficulty, and so on.
  • Syno® 3.0 high-throughput DNA synthesis platform, with prices as low as $0.09/bp.
  • Ensure that the delivered gene sequence is 100% accurate.
  • Our patented NGTM Codon gene optimization software provides optimization for free and significantly improves protein expression levels.

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Synthetic DNA Variant Library Synthesis Service

Synthetic DNA library synthesis is widely applied mechanism within various fields of research such as: discovery biology, protein engineering, structural biology, and gene expression. All of these fields of scientific research have revolutionized the world we live in today, through various means. One of the more popular discoveries in the genetics research community was the construction of the first synthetic DNA library. A synthetic DNA library is a pool of DNA fragm ents which is stored within a host micro-organism via molecular cloning. The resulting synthetic DNA library encodes variants of a common design and structure. Each fragment is stored in a separate host organism, such as bacterium. This offers a palpable advantage due to bacteria’s ability to quickly and accurately replicate the DNA to produce detectable quantities. This process allows us to have quick access to many different sequences of interest with ease and efficiency.

Compared with traditional methods for DNA synthesis

The traditional method of “design-build-test” routine to reach a genetic design to improve synthetic biology has been proven to be slow, poor, and unreliable. Due to this reason, an alternative method of generating a DNA libraries was discovered and utilized within the research community with higher frequency. With the Synthetic DNA library synthesis service, high performing genetic solutions will be worked out with ease and efficiency. The DNA library synthesis pool can achieve gene synthesis and cloning with a much faster, smarter, and more reliable approach. This allows for a much more accurate and efficient DNA library to be synthesized with the use of DNA synthesis. With a more accurate and efficient DNA library, various topics within genetics research will better understood and more effectively studied.

Synthetic DNA library synthesis makes biological engineering much more efficient

With DNA library synthesis based methods, highly complex sequences within synthetic biology can be engineered rapidly with high accuracy. It is a novel approach which allows for the customer specific DNA sequence to be more reliably synthesized and obtained with ease and higher accuracy. This also enables new biological systems and products to move toward potential revolutionary discoveries.

Custom Synthetic DNA library synthesis services

DNA synthesis allows us to synthesize gene libraries of any size and complexity, specific to the customer’s specifications. DNA library synthesis relies upon DNA synthesis, a method mastered by Synbio Technologies through our three phase Syno® Platform. This platform allows us to generate the customer specified DNA sequence of interest, up to and including 150 Kb in length, with one hundred percent accuracy. DNA synthesis is the technology that provides the foundation to meet the needs of the DNA library construction and maintenance. This strong foundation is important because DNA synthesis is the first step for generating DNA libraries. With the proper construction of DNA libraries researchers aim to identify novel genes and how each gene relates to various protein functions and structures.

DNA Synthesis Related Services

  • Codon Optimization
  • gene synthesis
  • Small Genome Synthesis
  • Pathway Synthesis

Gene Synthesis Services

At Synbio Technologies we offer a unique approach to gene synthesis services through our revolutionary Syno Gene Synthesis Platform. This three phase platform allows us to generate any customer specified genetic sequence in text format, up to and including 150 Kb in length. The requested sequences can vary greatly in both length and structure. For example, a requested sequence can contain a gene with a deleterious mutation in order to induce a debilitating effect, rendering the gene inactive in order to study the phenotypic effects. Or the requested sequence can be one not previously existing within nature, in order to study the effects of evolution or antibiotic resistance. These examples are made possible through Synbio Technology’s various gene synthesis services. First, our Syno® 2.0 Gene Synthesis plays a large role in small gene/short sequence synthesis. The Syno® 2.0 Gene Synthesis platform may be meant for short sequence synthesis, but it still has the capabilities and confidence of generating sequences with extreme accuracy of up to and including 150 Kb in length. Second, our Syno® 3.0 Next Generation Gene Synthesis Platform is a groundbreaking piece of technology that allows us to synthesize sequences that span near the length of small genomes. This gene synthesis services allows us to generate sequences up to an including 150 Kb in length, which was previously thought to be impossible to successfully and accurately engineer in a laboratory setting. With these two gene synthesis services, along with a wide range of other biological technology services, Synbio Technology is ready to supply our customers with the highest quality product within an efficient timeframe. This confidence starts with one of our most successfully gene synthesis services, the Syno® 2.0 Platform.

The Syno® 2.0 Gene Synthesis Platform is one of the corner stones of our gene synthesis services. This platform has various specializations in gene synthesis such as: pathway synthesis, small genome synthesis, vector construction, and codon optimization technology. With this platform, we are able to successfully and accurately synthesize the customer specified sequence of interest with one hundred percent accuracy. This is accuracy and confidence in sequence validity comes from the multiple occasions in which we use Sanger sequencing to verify the sequence being generated. In addition to the high quality sequence synthesis, we offer cloning of each prepared sequence to ensure the customer is provided with the adequate amount of product. Before each sequence is amplified, it is verified again with Sanger sequencing to verify the accuracy of the sequence. In addition to the specializations Syno® 2.0 offers, this platform also offers the ability to generate various structures of DNA commonly utilized in various fields of genetics research. Some of these structures include: hairpin structures, repeated sequences, high GC content regions, high AT content regions, or multiple consecutive nucleotide sequences. All of these structures offer a unique approach to studying various topics within the field of genetics. With the Syno® 2.0 Platform, and our other gene synthesis services, Synbio Technologies is ready to offer our customers a unique approach to conducting genetic research.

The Syno® 3.0 Next Generation Gene Synthesis Platform differs slightly from the Syno® 2.0 Platform, and our other gene synthesis services, but the output is still guaranteed to be one hundred percent accurate with a low price and fast turnaround time. The Syno® 3.0 Platform specializes in ChipBased DNA synthesis, CRISPR-Cas9 Libraries, and digital information storage. In other words, the Syno® 3.0 Platform is one of our gene synthesis services that offer a large amount of generated sequences to be synthesized. The chip-based gene synthesis approach allows us to generate thousands upon thousands of sequences all within a single chip. This allows for rapid production of sequences with high accuracy when multiple chips are used. The generated sequences are then, of course, verified with Sanger sequencing to guarantee one hundred percent accuracy. This method is used to generate large genes, up to and including 150 Kb in length with one hundred percent accuracy. In addition to this, the Syno® 3.0 platform gene synthesis services that Synbio Technologies offers, is the use of and experience with CRISPR-Cas9. Ever since the discovery and first successful utilization of CRISPR-Cas9, in 1993, it has been used time and time again in order to conduct genome edits. CRISPR-Cas9 has become one of the most popular and effective methods in order to conduct genome edits. This gene synthesis service offers CRISPR-Cas9 based gene knockout-out, gene knock-in, transcription activation, and transcription inhibition. All of these applications are methods of altering the expression level of a particular sequence/gene of interest. This alteration of expression will then be applied to various types of genetics research.

All of the gene synthesis services, as much as they vary in application, offered by Synbio Technologies have been tested and proven to be one hundred percent effective. This comes in the form of our guaranteed sequence validation, through Sanger sequencing. This accuracy and validity have given Synbio Technologies a reputation as one of the premier companies in the gene synthesis industries. This reputation relies heavily on our Syno® Gene Synthesis Platform. With this gene synthesis service platform, we are able to synthesize the sequence/gene of interest with extreme accuracy in a cost effective manner. This cost effective manner, on top of the efficient turnaround time, we at Synbio Technologies are ready to supply our customers with the highest quality product for the lowest prices.

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Gene Synthesis Protein Expression

At Synbio Technologies, we pride ourselves as being on the premier companies in the gene synthesis industry. Gene synthesis is defined as the method of synthetic biology that is used to engineer artificial genes in a laboratory setting. This technology has revolutionized the field of genetics, but can still have one small set back. This setback comes in the form of gene synthesis protein expression. One major problem is generating the necessary amount of protein expression critical in order to study various topics within the field of genetics. This problem arises from various predicaments that make certain aspects of research almost unbearable. A solution that Synbio Technologies offers is our gene synthesis protein expression package. Through the use of this package, we can guarantee up to 5 mg with 85% purity of the requested protein in a time efficient manner. At Synbio Technologies we offer a unique and revolutionary method of generating a customer requested sequence through the use of our Syno Gene Synthesis Platform. This three phase platform allows us to generate any requested genetic sequence, up to and including 150 Kb in length. With this platform we are more than capable of generating the requested genetic sequence while optimizing the gene synthesis protein expression along the way.

The gene synthesis protein package that Synbio Technologies has to offer has many cost effective and efficiency benefits when comparing to the “do it yourself” process. The overall process that Synbio Technologies has in place for optimizing gene synthesis protein expression includes: top-notch codon optimization, gene synthesis, protein expression, and 2-step purification. This process guarantees the highest quality product to our customers. Contrary to popular belief, this high quality product does not come with a large price tag, especially when compare to the “do it yourself” method. On average, our prices are over $1,000 cheaper, even when gene synthesis with codon optimization are included. This low price also comes with a much faster turnaround time, on average Synbio Technologies will have your final product prepared and shipped to your location three weeks faster than our customers who prefer the “do it yourself” method. One of the main reasons that Synbio Technologies is able to offer such ease in this aspect is through our use of the Syno Platform. The Syno Platform is the basis for increasing the resulting protein product with such accuracy in a quick turnaround time. The Syno Platform allows the customer to request a sequence of interest, in which we will generate a sufficient amount of the physical copy of the sequence with one hundred percent accuracy. This accuracy relies on multiple occasions of Sanger sequencing to verify the sequence validity after being generated and before amplification. This verification ensures that the highest quality product is shipped to our customers. The resulting sequence will then be put through expression tests in order to optimize the synthesized gene’s expression levels. These assessments guarantee the expression level will be up to par with the customer’s request. Once this is met, the protein is then purified to once again optimize the resulting product for later protein expression. The resulting final product is then shipped to the customer requested address. This process seems laborious, but at Synbio Technologies we offer quick turnaround times with the entire process from requested sequence to resulting protein at the customer’s location lasting six weeks.

With Synbio Technologies, accomplishing the proper gene synthesis expression level will be done with ease and efficiency in order to suite our customer’s request. The sequence of interest will be synthesized with one hundred percent accuracy, purified and optimized for the correct expression level. This confidence is relying upon Synbio Technology’s gene synthesis protein expression package. This package allows us to generate the requested amount of protein product and ship it to our customer’s within an efficient timeframe. This gene synthesis expression package has many advantages over the “do it yourself” process of generating the protein of interest, along with a smaller price tag. For these reasons, it is clear the Synbio Technologies is an optimum solution to all of our customer’s gene synthesis expression needs.

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Gene Synthesis Plasmid Preparation

At Synbio Technologies we pride ourselves in being one of the premier companies within not only the gene synthesis industry, but the biotechnology industry as a whole. This confidence is relying upon our tested and proven methods of gene synthesis. Gene synthesis can be defined as the method in synthetic biology used to engineer an artificial gene of interest in a laboratory setting. Ever since gene synthesis was first successfully conducted in the early 1970s it has become a highly sought mechanism in various fields of genetics research. For this reason many companies, like Synbio Technologies, have been attempting to optimize their gene synthesis products; but none of them have done this with quite success like Synbio Technologies. Our Syno Platform allows us to generate any sequence of interest up to and including 150 Kb in length. Another aspect of gene synthesis that Synbio Technologies has to offer is our gene synthesis plasmid preparation. Using this technology we are capable of generating any sequence and inserting it into any plasmid of interest. For gene synthesis plasmid preparation there are typically two forms of final product: transfection grade and research grade. Both differ slightly in their mechanism of being generated, as well as research application. Through use of Synbio Technology’s Syno® Platform, as well as our gene synthesis plasmid preparation we are ready to supply our customers with whichever plasmid product is necessary.

The differences between the transfection grade and research grade generated plasmids lies mainly in the applications each particular plasmid is used for. First, the research grade is used more in a laboratory setting to conduct various types of genetics research. These topics include: molecular cloning, mutagenesis, southern blotting, etc. All of these methods are used in order to conduct various types of genetics research with use of gene synthesis. Where gene synthesis comes in is through the insertion of the requested sequence into the plasmid. This insertion, and subsequent amplification, allows our customers to conduct any type of research necessary that requires a research grade plasmid construction. Second, the transfection grade plasmid is used mainly for protein manufacturing, antibody production and other forms of gene therapy. The link between this plasmid and gene synthesis is again the insertion of the sequence of interest into the particular plasmid. This can be extremely useful when conducting different types of gene therapy. The requested sequence can be loaded into the plasmid with the hopes of reversing the endogenous mutated gene of interest. This powerful technology has the ability to further our understanding of genetics and it is all thanks to gene synthesis.

Gene synthesis is something that Synbio Technologies does extremely well, mainly relying upon our Syno® Gene Synthesis Platform. This platform is used to generate the customer requested sequence with one hundred percent accuracy. The resulting gene synthesis product can then be used in order to prepare a plasmid of interest. This process is normally quite daunting, but Synbio Technologies is more than confident in our ability to generate the requested gene synthesis product and resulting gene synthesis plasmid. This process is all done within a quick turnaround time and competitive prices. For this reason, Synbio Technologies has risen to the top of not only the gene synthesis industry, but the resulting biotechnology industry as well. We offer a one stop shop for our customer’s gene synthesis plasmid preparation, with competitive prices, high quality output, and an efficient turnaround time. With this combination, our customers will be conducting research in no time with confidence in the product that we supply them.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Definition

The traditional method of studying a gene’s function is to extract the gene of interest, amplify it and put the resulting sequence through various molecular assessments. These assessments can vary from generating a frameshift mutation within the sequence, to upregulating the expression of the sequence in order to observe the resulting phenotypes the mutated sequence has induced on the organism. All of these methods are particularly important within the field of genetics research, but the methods of extracting these sequences to then mutate and study is becoming laborious and cumbersome. One, relatively recent and groundbreaking method, gene synthesis, has taken this traditional method of studying genetics and introduced some interest alterations. Gene synthesis is defined as the synthetic biology method used to engineer artificial genes within a laboratory setting. This method has allowed us, at Synbio Technologies, to go from a sequence in text format to physical copy of the genetic sequence itself with ease and extreme accuracy. Ever since gene synthesis was successfully conducted in 1972, by Hard Gobind Khorana and associates, this technology has been growing not only in popularity but in effectiveness and efficiency. One of the main reasons this technology has increased in popularity is that it allows for the creation of long, up to 150 Kb, sequences to be synthesized with extremely high accuracy. With the capability of generating sequences this large, gene synthesis gives a powerful approach to studying various topics such as complex pathways and generating synthetic DNA libraries. This high accuracy and relatively long length have promoted gene synthesis to be applied to various fields of genetics and biological research topics.

The fields of biological research that gene synthesis has been applied to vary from targeted mutagenesis in order to study a gene’s function to agriculture through increasing crop production. One of the main advantages that gene synthesis offers it the ability for a customer to request a specific sequence of DNA in text format up to 150 Kb in length. This text format is then converted into a physical copy with relative ease and high accuracy. The resulting sequence is then amplified an adequate amount of times in order to comply with the customer’s request. The accuracy allows the customer to generate a mutation anywhere within a gene of interest specific to their research topics. Not only can the mutation be generated anywhere, but any type of mutation can be used: frameshift, deamination, deletion, insertion, or nonsense mutation. In addition to this, gene synthesis can also be used to upregulate a gene of interest in order to cause an overexpression of the resulting phenotype. All of these mutations can be requested and inserted or applied the sequence of interest in order to obtain a better understanding of different types of genetics research such as evolution, or antibiotic resistance. In addition to these applications, gene synthesis has also been applied to agriculture through use of genetic engineering. Gene synthesis is the basis of genetic engineering, allowing for the creation of a resistance gene to a particular bacteria for example. The advantage that gene synthesis has to offer here is the ability to generate mutations within the synthesized gene to keep up with the constantly evolving bacteria. Gene synthesis through genetic engineering, have helped grow the agriculture industry, as well as develop a better understanding on how to improve crop survivability in varying climates as well as exposure to certain harmful bacteria. These applications have large impacts on not only the field of genetics, but the world. This is the main reason as to why Synbio Technologies is proud to be one of the premier companies within the gene synthesis industry.

At Synbio Technologies, we try to keep our gene synthesis process as simple as its definition. Gene synthesis is carried out with ease and extremely high accuracy through our Syno® Gene Synthesis Platform. This three phase platform allows us to generate the physical genetic sequence of the customer specified request. This generated sequences is guaranteed to be one hundred percent identical to the customer requested sequence. The guarantee is backed by Sanger sequencing at multiple phases of the three platforms. The resulting gene synthesis product will then be shipped to the customer specific location within an efficient timeframe. This entire process allows Synbio Technologies to offer the highest quality product, with an efficient turnaround time. These two aspects, combined with competitive prices and respectable reputation, it is clear that Synbio Technologies is one of the premier companies within the gene synthesis industry.

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Large Gene Synthesis

An interesting and important aspect of genetic research that has grown drastically in popularity over the last ten to fifteen years is gene synthesis . Gene synthesis can be defined as the construction of a physical genetic sequence from a requested sequence in text format. Gene synthesis allows us, at Synbio Technologies, to manufacture genetic sequences that may not already exist within nature. In addition to the de novo sequences generated, sequences can also vary greatly in length, from short sequences to large sequences up to 150 Kb. This process is carried out with Synbio Technology’s, patent pending, Syno® Platform. This three stage platform makes going from requested costumer specific sequence to physical copy of the sequence with ease. One aspect in particular that the Syno® Platform is known for is large gene synthesis. The Syno® platform has the ability to synthesize large genes up to and including 150 Kb in length. The wide range of possibilities makes Synbio Technology’s Syno® Platform attractive and sought out by researchers all over the world especially when it refers to large gene synthesis. This has led Synbio Technologies to become one of the premier companies within the realm of not only large gene synthesis, but any length gene synthesis. This confidence in our large gene synthesis relies upon one of the two most popular methods of gene synthesis, proven time and time again.

There are two common methods of large gene synthesis: Gibson Assembly Method and Yeast homologous recombination technology. The Gibson Assembly Method now the most common method of achieving directed cloning and in vitro multiple-segment assembly. The Gibson Assembly Method is commonly used by various companies to conduct gene synthesis for varying lengths. It is an effective method for gene synthesis, but the one major restriction that comes along with this method is the inability to easily synthesize fragments over 20kb in length. This is an acceptable method for small gene synthesis, but a different method is needed for large gene synthesis. The yeast homologous recombination technology has the ability to assemble fragments up to 1.08Mb in length. This length is larger than the average prokaryotic genome, making yeast homologous recombination technology a reliable method to use for both large and small gene synthesis. It is yeast homologous recombination that is utilized for Synbio Technology’s Syno® 2.0 and Syno® 3.0 platforms, the foundation of gene synthesis at Synbio Technologies. It is this technology that allows us to provide one-stop services which includes: basic gene synthesis, gene cluster, and large gene synthesis. With this technology, the Syno® Platforms not only makes it possible to synthesize large genes, but small genomes as well. In addition to the large gene synthesis, Synbio Technology also offers the ability to guarantee 100% sequence verification. This is carried out using Sanger sequencing at multiple steps of the Syno® Platform stages in order to verify the sequence accuracy. The sequencing verification is necessary when dealing with large gene synthesis. The accuracy of sequences of this length is not always guaranteed to be one hundred percent to the identical sequence with other companies, but with our Syno® Platform it is.

At Synbio Technologies we are more than confident in our abilities to generate your requested genetic sequence, up to and including 150 Kb in length. The possibility of synthesizing a gene this large has opened up a vast amount of new opportunities and approaches to various topics within genetics research. Large gene synthesis is not a task that is easily accomplished, but at Synbio Technologies we are capable of generating the requested sequence with one hundred percent accuracy in an efficient timeframe. This confidence is relying upon the efficiency and accuracy of our Syno® Platform and how it has been proved its ability to generate genetic sequences up to 150 Kb. In addition to this, our prices are at an all-time low, starting at $0.19 per base pair through our gene synthesis promotions. With this confidence in our large gene synthesis protocol and low prices we are more than ready to offer you the highest quality product for an extremely competitive price.

Gene Synthesis Related Services

  • Codon Optimization
  • Vector Construction
  • Small Genome Synthesis
  • Pathway Synthesis

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Vectors

A relatively recent discovery, dating back to the early 1970s, in biology has revolutionized the field of genetics research. This discovery is known as gene synthesis, which allows for the manufacturing of a physical genetic sequence from a string of base pairs in text format. Gene synthesis allows for the creation of a genetic sequence that may not be preexisting within nature. Previously, a sequence of interest was required to be present within an organism in order to be extracted and later amplified to study its function. Gene synthesis allows us, at Synbio Technologies, to bypass this requirement entirely. This is accomplished through our, patent pending, Syno® Platform, which allows us to move from a sequence in text format to a physical copy with ease and accuracy. One common advantage that gene synthesis offers is the construction of gene synthesis vectors. Gene synthesis vectors vary widely in structure, function, length, and physical sequence characteristics. With the use of Synbio Technology’s Syno® Platform we are capable of not only synthesizing any gene of interest, up to 150 Kb in length, but constructing any gene synthesis vector specified by the customer. The generated gene synthesis vector can then be used in a wide range of applications, specific to each type of gene synthesis vector.

There are three main types of gene synthesis vectors that Synbio Technologies prides itself in its ability to generate with high accuracy in order to better suite the customer’s particular request. The three vectors are: short hairpin RNA (shRNA), transcription activator-line effector nuclease (TALEN), and CRISPR-Cas9. All three have similar functions, gene knockout also known as gene silencing, but have various methods in order to accomplish this. shRNA use RNA interference in order to block the transcription and translation of the gene/sequence of interest, rending it inactive. TALEN and CRISP-Cas9 use genome editing in order to render the gene/sequence of interest inactive. All three have been tested and proven as effective methods of gene silencing and inhibiting the resulting protein expression. Gene silencing is a common method within genetics research in order to determine the function of a gene. Once the gene is rendered inactive, researchers can study the resulting phenotype and infer on the function of gene of interest. Where gene synthesis comes into play is through our Syno® 2.0 and Syno® 3.0 Platforms Synbio Technologies is capable of constructing the requested sequence within any of these vectors, in addition to other popular vectors. This technology allows the customer to design a sequence, in text format, which allows Synbio Technologies to generate the requested gene synthesis vector. Once generated, the sequence and resulting vector will work to inhibit the endogenous gene, lowering protein expression and essentially silencing the gene of interest. As mentioned before, this silencing is achieved through the various methods of gene silencing utilized by each vector.

At Synbio Technologies we are extremely confident in our ability to construct these gene synthesis vectors to the customer’s exact specifications. We guarantee with one hundred percent accuracy within our generated sequences in order to produce the highest quality output. With this accuracy we can guarantee the best possible method of achieving a gene knockout through the use of our gene synthesis vectors. At Synbio Technologies we don’t only offer the highest quality product, but extremely fast turnaround times and competitive prices as well. Within as few as 10 days the customer’s gene synthesis vector will be constructed and be shipped to the customer specified location. With this confidence, high quality output, fast turnaround time, and competitive prices it is clear the Synbio Technologies is one of the leading companies within the gene synthesis industry.

Gene Synthesis Related Services