Using CRISPR/Cas9 for genome editing, allowing to precisely edit the genome of cells by inducing double-stranded DNA (dsDNA) breaks at specific loci, is both an efficient and cost-effective technological tool. sgRNA serves as a guide to recognize and combine target with DNAs, allowing activated Cas9 nuclease to attach, cleave, and modify the PAM downstream target DNA. CRISPR-based genome editing technology has made it easier and faster than ever to alter a specific DNA sequence in the genome or perform genome-wide functional screening tests to identify genes involved in a particular phenotype. CRISPR-Cas9 is considered the most promising tool in gene modification. Our design center provides single sequence sgRNA design or whole-genome sgRNA library design, downstream verification, and stable cell line construction services. We offer a one-stop solution for CRISPR-Cas9 projects to achieve high genome editing efficiency.
Abbreviations:
PAM: Protospacer Adjacent Motif
DSB: Double Strand Break
NHEJ: Non-Homologous End Joining
HDR: Homology Directed Repair
GFP: Green Fluorescent Protein
sgRNA Library Design
Synbio Technologies offers custom sgRNA design services to help researchers achieve their gene editing goals. We have a team of experienced scientists who can help you choose the best sgRNA for your experiment and design a custom sequence that will achieve the desired editing outcome. We also offer a range of additional services, including vector construction, cloning, and protein expression, so you can get your experiment up and running as quickly as possible.
With over a decade of experience in the field of synthetic biology, Synbio Technologies is your trusted partner for all your synthetic biology needs.
Reasons to partner with Synbio Technologies
- Design: Providing single sgRNA, multiple sgRNA, and sgRNA library design services.
- Experience: Well-designed sequences with lowest off-target rate, with professional analysis on every project.
- Variety: Design for >20 different species. Custom and specific design is available upon request.
- Customized Vectors: sgRNA can be designed in any vector that meets your needs.
