CRISPR Amplicon Sequencing

  • DNA Editing
  • CRISPR-Cas9
    sgRNA Design
    sgRNA Library Construction
    Human Genome Knockout Libraries
    sgRNA Panel
    Microbial Genome Editing
    Mammalian Cell Genome Editing
    ssDNA Synthesis
    CRISPR Amplicon Sequencing
    CRISPR NGS Data Analysis
  • DNA Reading
  • Hybridoma Antibody Sequencing
    Immune Repertoire Sequencing
    DNA Sequencing & Gene Analysis
    NGS High Throughput Sequencing
    16S rDNA Sequencing
    Metagenome Sequencing
    RNA Sequencing
    CRISPR Amplicon Sequencing

    After the fragments encoding Cas9 and sgRNA are transfected into cells through plasmids or other means, Cas9 cuts the target genome at a specific site, causing DNA double-strand break (DSB), which is joined through nonhomologous end joining (NHEJ) or homologous recombination (HR), inserts or deletes (indel) of DNA, or relies on a homologous template containing specific mutations for targeted modification of the target site. Synbio Technologies uses amplicon sequencing to test the results of CRISPR/Cas9 genome editing, as well as to analyze off-target effects, which helps researchers quickly locate gene mutation sites and study gene functions.

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    CRISPR Amplicon Sequencing

    Competitive Advantages

    • High Coverage: Each reaction can perform multiplex analysis of hundreds to thousands of amplicons.
    • High Flexibility: It can be used for all kinds of mutation verification and screening genetic variation.
    • Highly Customized: Analysis services can be customized.
    • Cost-effective: Compared with whole-genome sequencing, it can reduce sequencing cost and turnaround time.

    CRISPR Amplicon Sequencing Process

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    Service Specification

    Amplicon SizePlatform ConfigurationPriceTurnaround TimeDeliverables
    50-150 bpIllumina 2×150 bpQuote3-4 weeks
    • Sequencing chromatogram
    • Data analysis report
    150-350 bpIllumina 2×250 bp4-5 weeks
    >350 bp MiSeq 2×300 bpQuote

    * For more information please contact us at quote@synbio-tech.com.

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