CRISPR Amplicon Sequencing

After the fragments encoding Cas9 and sgRNA are transfected into cells through plasmids or other means, Cas9 cuts the target genome at a specific site, causing DNA double-strand break (DSB), which is joined through nonhomologous end joining (NHEJ) or homologous recombination (HR), inserts or deletes (indel) of DNA, or relies on a homologous template containing specific mutations for targeted modification of the target site. Synbio Technologies uses amplicon sequencing to test the results of CRISPR/Cas9 genome editing, as well as to analyze off-target effects, which helps researchers quickly locate gene mutation sites and study gene functions.

crispr-amplicon-sequencing-0928

CRISPR Amplicon Sequencing

Competitive Advantages

  • High Coverage: Each reaction can perform multiplex analysis of hundreds to thousands of amplicons.
  • High Flexibility: It can be used for all kinds of mutation verification and screening genetic variation.
  • Highly Customized: Analysis services can be customized.
  • Cost-effective: Compared with whole-genome sequencing, it can reduce sequencing cost and turnaround time.

CRISPR Amplicon Sequencing Process

crispr-amplicon-sequencing-process

Service Specification

Amplicon SizePlatform ConfigurationPriceTurnaround TimeDeliverables
50-150 bpIllumina 2×150 bpQuote3-4 weeks
  • Sequencing chromatogram
  • Data analysis report
150-350 bpIllumina 2×250 bp4-5 weeks
>350 bp MiSeq 2×300 bpQuote

* For more information please contact us at quote@synbio-tech.com.

Related Services