CRISPR-Cas9 sgRNA Panel

Due to its simplicity and ease of customization, the CRISPR-Cas9 gene editing system is a popular tool in genetic screening. Recently, more and more literature report that CRISPR-Cas9 is more effective, reliable, and specific than RNAi in gene knockout experiments. Gene knockout screening utilizing CRISPR-Cas9 has been widely used for target and gene identification in many research areas, such as cancer development, drug resistance, immune response, bacterial toxin susceptibility, etc.

With our proprietary CRISPR-Cas9 sgRNA design platform, Synbio Technologies has designed 10 sgRNAs for each target gene, and constructed the CRISPR-Cas9 sgRNA Panel libraries. The delivery of these libraries includes plasmid, lentivirus, ready-to-use sgRNA, and more.

We provide integrated sgRNA for every gene in panels promoting cancer development and evolution, cell cycle pathway target screening, and cell pathway genetics screening research.

CRISPR-Cas9 sgRNA Panel Competitive Advantages

  • Customized Design: Highly customizable sgRNA panel design for any variety of target genes.
  • Flexible Delivery Format: Including plasmid, lentivirus, ready-to-use sgRNA, etc.
  • Proprietary DNA Synthesis Platform: Fast and efficient sgRNA panel construction.

CRISPR-Cas9 sgRNA Panel Screening Workflow

Available methods for lentivirus CRISPR-Cas9 sgRNA library screening includes screening of resistance targets against the following: the anti-cancer drug Vemurafenib, the anti-α-toxin of Clostridium botulinum, and anti 6-sulfur guanine. Pooled Panel library screening, combined with NGS technology, can accurately identify the target genes affected by the screening condition(s). Synbio Technologies offers two of the most common types of library screening: positive screening and negative screening.

sgrna-panel-screening-workflow

Screen TypeMethod of Use
Postive/Forward Screening
  • Application of sgRNA Panel
  • Screening condition: most cells dead or failed to transfect into plasmid or virus
  • Selection of surviving cells
  • NGS of the surviving cells
  • Obtaining the gene list related to the screening condition
Negative/Reverse Screening
  • Application of sgRNA Panel
  • Perform next generation sequencing on control sample(no selection):in parallel, apply library
  • Screening condition: most cells survived
  • NGS of the surviving cells
  • Sequencing result comparison between two groups of cells
  • Determine the sgRNA that disappeared due to the insertion of screening condition

Human Protein Kinase sgRNA Panel

Synbio Technologies has developed sgRNA Panels for human protein kinase genes, including genes which regulate cell pathway, cell cycle, and cell signal transduction. Due to the sequence homology of the catalytic domain, over 92% of human protein kinase belongs to a huge category of protein kinases, which includes 9 different protein kinase families.

Protien Kinase FamiliesProtien Kinases
AGC Protein Kinase FamilyPKA, PKC, PKG, etc.
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CAMK Protein Kinase FamilyCalcium dependent and calmodulin dependent protein kinase
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CK1 Protein Kinase FamilyCasein kinase 1, etc.
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CMGC Protein Kinase FamilyCDK, MAPK, GSK3, CLK, etc.
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STE Protein Kinase FamilyYeast sterile7/11/20 protein kinase homologous analogues, etc.
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TK Protein Kinase FamilyTyrosine protein kinase, etc.
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TKL Protein Kinase FamilyTyrosine like protein kinase, etc.
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Atypical Protein Kinase FamilyADCK, Brd, etc.
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Other Protein Kinase FamilyNEK, Wnk, etc.
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