CRISPR-Cas9 sgRNA Panel

  • DNA Editing
  • CRISPR-Cas9
    sgRNA Design
    sgRNA Library Construction
    Human Genome Knockout Libraries
    sgRNA Panel
    Microbial Genome Editing
    Mammalian Cell Genome Editing
    ssDNA Synthesis
    CRISPR Amplicon Sequencing
    CRISPR NGS Data Analysis

    Due to its simplicity and ease of customization, the CRISPR-Cas9 gene editing system is a popular tool in genetic screening. With our proprietary CRISPR-Cas9 sgRNA design platform, Synbio Technologies has designed 10 sgRNAs for each target gene, and constructed the CRISPR-Cas9 sgRNA Panel libraries. The delivery of these libraries includes plasmid, lentivirus, ready-to-use sgRNA, and more. We provide integrated sgRNA for every gene in panels designed for cancer development and evolution, cell cycle pathway target screening, and cell pathway genetics screening research, etc.

    CRISPR-Cas9 sgRNA Panel Competitive Advantages

    • Customized Design: Highly customizable sgRNA panel design for any variety of target genes.
    • Flexible Delivery Format: Including plasmid, lentivirus, ready-to-use sgRNA, etc.
    • Proprietary DNA Synthesis Platform: Fast and efficient sgRNA panel construction.

    CRISPR-Cas9 sgRNA Panel Screening Workflow

    Available methods for lentivirus CRISPR-Cas9 sgRNA library screening includes screening of resistance targets against the following: the anti-cancer drug Vemurafenib, the anti-α-toxin of Clostridium botulinum, and anti 6-sulfur guanine. Pooled Panel library screening, combined with NGS technology, can accurately identify the target genes affected by the screening condition(s). Synbio Technologies offers two of the most common types of library screening: positive screening and negative screening.

    sgrna-panel-screening-workflow

    Screen TypeMethod of Use
    Postive/Forward Screening
    • Application of sgRNA Panel
    • Screening condition: most cells dead or failed to transfect into plasmid or virus
    • Selection of surviving cells
    • NGS of the surviving cells
    • Obtaining the gene list related to the screening condition
    Negative/Reverse Screening
    • Application of sgRNA Panel
    • Perform next generation sequencing on control sample(no selection):in parallel, apply library
    • Screening condition: most cells survived
    • NGS of the surviving cells
    • Sequencing result comparison between two groups of cells
    • Determine the sgRNA that disappeared due to the insertion of screening condition

    Human Protein Kinase sgRNA Panel

    Synbio Technologies has developed sgRNA Panels for human protein kinase genes, including genes which regulate cell pathway, cell cycle, and cell signal transduction. Due to the sequence homology of the catalytic domain, over 92% of human protein kinase belongs to a huge category of protein kinases, which includes 9 different protein kinase families.

    Protien Kinase FamiliesProtien Kinases
    AGC Protein Kinase FamilyPKA, PKC, PKG, etc.
    Please click here to check the whole list
    CAMK Protein Kinase FamilyCalcium dependent and calmodulin dependent protein kinase
    Please click here to check the whole list
    CK1 Protein Kinase FamilyCasein kinase 1, etc.
    Please click here to check the whole list
    CMGC Protein Kinase FamilyCDK, MAPK, GSK3, CLK, etc.
    Please click here to check the whole list
    STE Protein Kinase FamilyYeast sterile7/11/20 protein kinase homologous analogues, etc.
    Please click here to check the whole list
    TK Protein Kinase FamilyTyrosine protein kinase, etc.
    Please click here to check the whole list
    TKL Protein Kinase FamilyTyrosine like protein kinase, etc.
    Please click here to check the whole list
    Atypical Protein Kinase FamilyADCK, Brd, etc.
    Please click here to check the whole list
    Other Protein Kinase FamilyNEK, Wnk, etc.
    Please click here to check the whole list