Genome Editing Service in Mammalian Cells

  • DNA Editing
  • CRISPR-Cas9
    sgRNA Design
    sgRNA Library Construction
    sgRNA Panel
    Microbial Genome Editing
    Mammalian Cell Genome Editing
    ssDNA Synthesis
    CRISPR Amplicon Sequencing
    CRISPR NGS Data Analysis

    Depending on the effectiveness and simplicity, the CRISPR-Cas9 system has become the most commonly utilized to conduct genome editing within mammalian research. Synbio Technologies can provide genome editing in mammalian cells, including CRISPR-Cas9 sgRNA design, synthesis, activity detection, package into lentivirus, transfer into cells and specific gene knock in/out. With this combination of various services, we are confident in our ability to provide our customers with a specific approach to accomplish their research goals.

    Mammalian Genome Editing Service Process

    genome-editing-in-mammalian-cells

    Competitive Advantages

    • Wide Range of Applications: No restrictions among genomic sequences, cell types, or species.
    • Simple and Convenient to Construct: Only a short sequence, approximately 20bp in length, of the sgRNA sequence to complete target recognition.
    • Efficient Turnaround Time: Genetic stability of homozygous strains can be constructed within an efficient timeframe.
    • Multiple Site Knock Out: Multiple sites can be targeted at the same time to successfully accomplish multi-site knockout.

    Mammalian Gene Editing Service Program

    sgRNA Endogenous Activity Detection

    1. A variety of sgRNA endogenous activity assays guarantee the efficiency of the generated gene knock-out.
    2. The methods of detection include: SSA activity detection, in vitro cleavage activity detection, and endogenous activity detection.

    Lentiviral Package

    Lentiviral package services offer: transfection of mammalian cells, facilitation of stable Cas9 protein-expressing cell lines, and improving gene knock-out efficiency.

    Small Animal Gene Knock-out

    With our experienced team of R&D engineers, Synbio Technologies is capable of providing gene knockout services for zebrafish, mouse, and rat genomes.

    CRISPR-Cas9 Genome Editing Frequently Asked Questions

    • What are the advantages of CRISPR-Cas9 genome editing? Read more
    • How was the CRISPR-Cas9 system found? Read more
    • How to apply the CRISPR-Cas9 system? Read more

    CRISPR-Cas9 Genome Editing Related Services

    References

    [1].Shen, B., et al., Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nature Methods, 2014. 11(4): p. 399-402.
    [2].Zou, Q., et al., Generation of gene-target dogs using CRISPR/Cas9 system. Journal of Molecular Cell Biology, 2015. 7(6).
    [3].Liang, P., et al., CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & Cell, 2015. 6(5): p. 363-372.