sgRNA Bioinformatics Service

Synbio Technologies provides professional sgRNA bioinformatics services for CRISPR-Cas9 system. The services we offer range from sgRNA design, synthesis and gene editing, to gene expression profiling analysis to chromosomal region with DNA copy number statistics based on sequencing results as functional analysis. Combined with professional databases such as PRECOG, TCGA and COSMIC, we can analyze gene expression levels, gene mutation rates and relevance of clinical prognosis. This allows us to provide solutions for potential drug targets and personalized medicine. It could also help scientists find more genes serve as therapeutic targets.

One-Stop Service for sgRNA

sgRNA Bioinformatics

Competitive Advantages

• Experienced in >20 species for CRISPR-Cas9 sgRNA design, which can be customized for specified requirements.
• Professional bioinformatics analysis team with scientific scheme, in-depth data mining to generate a reliable and flexible data report.

Applications

Agriculture, microbiology, environmental biology, oceanography, human microecology, etc.

Case Studies

The genomes cells were infected in different stages with viruses carrying sgRNA by CRISPR-Cas9 library. The cells were then sequenced with next-generation sequencing technology.

We first evaluated the effect of sgRNA knock out. Secondly, the enriched or deletion genes were analyzed and compared to screen the genes and targets for cell survival or drug action by functional annotations such as GO and KEGG . Finally, the targets were further analyzed by combining the cancer database and mutation database.

The results are as follows:

Service Specifications