sgRNA Bioinformatics Service

  • DNA Editing
    • CRISPR-Cas9
    sgRNA Design
    sgRNA Library Construction
    Human Genome Knockout Libraries
    sgRNA Panel
    Microbial Genome Editing
    Mammalian Cell Genome Editing
    ssDNA Synthesis
    CRISPR Amplicon Sequencing
    CRISPR NGS Data Analysis

    Synbio Technologies provides professional sgRNA bioinformatics services for CRISPR-Cas9 system. The services we offer range from sgRNA design, synthesis and gene editing, to gene expression profiling analysis to chromosomal region with DNA copy number statistics based on sequencing results as functional analysis. Combined with professional databases such as PRECOG, TCGA and COSMIC, we can analyze gene expression levels, gene mutation rates and relevance of clinical prognosis. This allows us to provide solutions for potential drug targets and personalized medicine. It could also help scientists find more genes serve as therapeutic targets.

    One-Stop Service for sgRNA

    sgRNA-bioinformatics-service

    sgRNA Bioinformatics

    sgRNA-bioinformatics

    Abbreviation:
    Alternative TSS: Alternative Transcription Start Sites

    Competitive Advantages

    • Experienced in >20 species for CRISPR-Cas9 sgRNA design, which can be customized for specified requirements.
    • Professional bioinformatics analysis team with scientific scheme, in-depth data mining to generate a reliable and flexible data report.

    Applications

    Agriculture, microbiology, environmental biology, oceanography, human microecology, etc.

    Case Studies

    The cells of different samples were infected with viruses carrying CRISPR-Cas9 sgRNA library. The cells were then sequenced with next-generation sequencing technology.
    We first evaluated the effect of sgRNA knock out. Secondly, the enriched or depleted sgRNAs were analyzed and compared to screen the genes and targets for cell survival or drug action by functional annotations such as GO and KEGG. Gene Set Enrichment Analysis is the process of finding a class of genes or proteins that are overexpressed in a group of genes or proteins. The common ones are GO functional annotation and KEGG pathway enrichment analysis. Through gene pathway enrichment analysis, we can preliminarily analyze the biological processes or signaling pathways that differentially expressed genes may participate in. Finally, the targets were further analyzed by combining the cancer database and mutation database.

    The results are as follows:

    PCA-analysis
    PCA analysis
    differentially-expressed-gene-volcano-plot
    Differentially expressed gene volcano plot
    effect-of-sgRNA-knock-out-on-gene-expression
    Effect of sgRNA knock-out on gene expression
    differentially-expressed-gene-venn-diagram
    Differentially expressed gene venn diagram
    KEGG-pathway-enrichment-plot
    KEGG pathway enrichment plot
    GO-enrichment-bubble-plot
    GO enrichment bubble plot
    Scatter-plot-of-beta-score
    Scatter plot of beta score
    TCGA-database
    Mining potential drug targets with data from TCGA database
    sites-of-exons
    Analysis of sgRNA on the knock out sites of exons

    Service Specifications

    ServiceAnalysis Analysis SoftwareTurnaround TimeDeliverablesPrice
    sgRNA Bioinformatics AnalysisGenome, Transcriptome, Drug Target Mining, etc.R software7-10 Business DaysAnalysis ReportQuote