Microbial Genome Editing

  • DNA Editing
  • CRISPR-Cas9
    sgRNA Design
    sgRNA Library Construction
    sgRNA Panel
    Microbial Genome Editing
    Mammalian Cell Genome Editing
    ssDNA Synthesis
    CRISPR Amplicon Sequencing
    CRISPR NGS Data Analysis

    As our understanding of DNA sequences (Genotype) and biological functions (Phenotype) increases, the ability to design and synthesize (Synotype) genes, pathways, genomes, and organisms by using synthetic biology techniques is also improving quickly. Synbio Technologies can provide a full range of DNA reading (sequencing), DNA writing (synthesis), and DNA editing services. Relying on our synthesis and assembly platform and CRISPR-Cas9 technology, Synbio Technologies can provide efficient editing of microbial genomes. Synbio Technologies has established CRISPR gene editing systems in more than dozens of bacteria (E.coli, Helicobacter pylori, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus aureus, etc.), fungi (Saccharomyces cerevisiae, Monascus, Aspergillus niger, Penicillium, Aspergillus fumigatus, etc.) and other species.

    Why us?

    • Applicable in Many Species: We provide genome editing services for bacteria, fungi, and other microorganisms.
    • Efficient Genome Editing Tool: Edit any gene at any locus with high accuracy.
    • Multiple Sites Mutant: Genes can be modified in multiple sites simultaneously.

    Service Specifications

    Microbial TypesService DetailsInformation Requested from ClientsTAT
    (Business Days)
    Deliverables
    • Bacteria
    • Yeast
    • Mold
    Construction of knock-out strains
    • Strain that needs to be modified along with strain information.
    • Information of the knock-out gene along with knock-out sequences. Or knock-in sites and replacement sequences.
    Inquire
    • Report (Reagents, instruments, experimental protocol, sequencing results, etc.)
    • Sequencing File
    • Edited Strains
      Note: plasmids for editing are not delivered.
    Construction of knock-in strains
    Construction of site directed mutagenesis stains

    Yeast Genome Editing Case Study

    The ADE1 gene controls expression of adenine in yeast. ADE1 mutant yeast were engineered through CRISPR-Cas9. These mutants exhibited an accumulation of red pigment, a phenotype associated with the lack of adenine.

    Fig.1. Pink yeast colonies which were programmed by CRISPR-Cas9 gene modification.
    Fig.2 DNA sequencing

    DNA sequencing indicates that 18 base pairs were missing (fig.2), suggested successful gene modification.