Recently, the CRISPR-Cas9 system has been used as an easy and cost-efficient method of genome modification in simple model organisms. Genome editing in yeast can help to produce efficient mutant yeast colonies and therefore promote their applications in different industries such as biopharmaceuticals, pollution control, energy, chemical bio-manufacturing, etc. Depending on the innovative “GPS” (Genotype, Phenotype and Synotype) platform, Synbio Technologies provides a one-stop platform for highly accurate editing and modification of the yeast genome.
- Efficient Genome Editing Tool: Edit any gene at any locus with high accuracy.
- Multiple Sites Mutant: Genes can be modified in multiple sites simultaneously.
- Fast Turnaround Time: 4-5 Weeks
|Services||Information Requested from Clients||Deliverables||Turnaround Time|
|Yeast gene knock-out services||Modified glycerol yeast and QC report||4-5 weeks|
|Yeast gene knock-in and replacement services||Modified glycerol yeast and QC report||4-5 weeks|
Yeast Genome Editing Case Study
The ADE1 gene controls expression of adenine in yeast. ADE1 mutant yeast were engineered through CRISPR-Cas9. These mutants exhibited an accumulation of red pigment, a phenotype associated with the lack of adenine.
DNA sequencing indicates that 18 base pairs were missing (fig.2), suggested successful gene modification.