Yeast Genome Editing

Recently, the CRISPR-Cas9 system has been used as an easy and cost-efficient method of genome modification in simple model organisms. Genome editing in yeast can help to produce efficient mutant yeast colonies and therefore promote their applications in different industries such as biopharmaceuticals, pollution control, energy, chemical bio-manufacturing, etc. Depending on the innovative “GPS” (Genotype, Phenotype and Synotype) platform, Synbio Technologies provides a one-stop platform for highly accurate editing and modification of the yeast genome.

Competitive Advantages

  • Efficient Genome Editing Tool: Edit any gene at any locus with high accuracy.
  • Multiple Sites Mutant: Genes can be modified in multiple sites simultaneously.
  • Fast Turnaround Time: 4-5 Weeks

Service Specifications

ServicesInformation Requested from ClientsDeliverablesTurnaround Time
Yeast gene knock-out services
  • Strain that needs to be modified along with strain information.
  • Information of the knock-out gene along with knock-out sequences.
Modified glycerol yeast and QC report4-5 weeks
Yeast gene knock-in and replacement services
  • Strain that needs to be modified along with strain information.
  • Information of the knock-out gene along with knock-out sequences.
  • Knock-in sites and replacement sequences.
Modified glycerol yeast and QC report4-5 weeks

Yeast Genome Editing Case Study

The ADE1 gene controls expression of adenine in yeast. ADE1 mutant yeast were engineered through CRISPR-Cas9. These mutants exhibited an accumulation of red pigment, a phenotype associated with the lack of adenine.

Fig.1. Pink yeast colonies which were programmed by CRISPR-Cas9 gene modification.
Fig.2 DNA sequencing

DNA sequencing indicates that 18 base pairs were missing (fig.2), suggested successful gene modification.