Detecting DNA Fragmentation for Apoptosis

Apoptosis is the autonomous and orderly death of cells controlled by genes to maintain the stability of the internal environment. These genes are very conservative among species, such as the Bcl-2 family, caspase family, and so on. One of the most important characteristics of apoptosis is the degradation of chromatin DNA. DNA Fragmentation is the main feature of apoptosis.

There are three common methods to detect DNA fragmentation: DNA ladder assays, TUNEL assays, and comet assays. These methods have their own characteristics and can be chosen under different experimental conditions. For example, DNA ladder assays are relatively easy to perform but needs lots of apoptotic cells, comet assays are inexpensive and rapid but do not allow distinguishing between genotoxicity and early apoptosis, while TUNEL assays are more sensitive but in situ is not a method specific only for apoptotic DNA fragmentation.

Synbio Technologies supplies our customers with double-stranded, sequence-verified Synstrands DNA Fragments in only a few business days. These fragments offer affordable and accessible construction or modification of genetic sequences. The various applications include antibody research and CRISPR-mediated gene/genome editing, being used as qPCR standards, etc. Our Synstrands DNA fragment synthesis service provides a more rapid and efficient method for simple gene synthesis and construction. Synstrands DNA Fragments are available in two delivery forms: Eppendorf tubes or plates.

Reference: https://doi.org/10.1007/s11033-018-4258-9

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