DNA Sequencing & Gene Analysis

Synbio Technologies provides reliable DNA sequencing services to scientific researchers and industrial customers around the world. We are qualified and professional in Sanger DNA Sequencing for normal biosynthesis and cloning projects. We also offer next generation sequencing (NGS) for the analysis of transcriptome and genome. Synbio Technologies has been certified with the highest manufacturing standards (ISO9001 & ISO13485). Our well-equipped and experienced technical staff guarantees you fast and reliable gene sequencing and analysis services.

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Why us?

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Reliability
Advanced sequencer, tidy FIG peaks, sequential reading lengths could reach 800bp

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Secrecy
Data uniqueness and full control over your own documents

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Integrity
Gold standard, comprehensive platform includign Sanger sequencing platform & NGS

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Professional
Technical support, 15+ years of sequencing and analysis experience

Case study

shRNA structure sequencing

shRNA design is typically divided into two formats, the simple stem-loop shRNA and the microRNA-adapted shRNA (Fig.1). Due to the hairpin structure, shRNA is difficult to sequence in gene synthesis. Universal sequencing protocols can especially hardly work for the second structure’s sequence (Fig.2). However, Synbio Technologies developed and enhanced the protocol, and obtained high quality sequencing result (Fig.3). With upgraded process and long-standing expertise, Synbio Technologies is always well good at dealing with difficult structures such as hairpin structure, poly structure, etc..

Fig.1 Centroid secondary structure
Fig.1 Centroid secondary structure

Conventional protocol result

shRNA template data using standard sequencing protocol
Fig.2 No correct sequencing results for the shRNA template

Synbio Technologies’ protocol result

Fig.3 Perfect results from Synbio upgraded sequencing program