Gene Synthesis by PCR

Prior to the 1970s traditional cloning was used in order to amplify a gene of interest. This process of gene synthesis by PCR is still used today, but relies on a laborious process of achieving the final amplified product. Traditional cloning relies on recombinant DNA being integrated into a bacteria host’s genome. Once the DNA is integrated into the bacteria host’s genome, the DNA is then amplified and later extracted. Unfortunately, this process allows for many variables to be interfered with resulting in a tarnished and lower quality product. In the early 1980s, Kary Mullis discovered and developed a much more efficient method of amplifying genes of interest. This method is called polymerase chain reaction (PCR). The basic process that PCR follows starts with the addition of two primers and a heat-stable Taq polymerase to the targeted gene. The mixture is then heated, allowing the hydrogen bonds between strands to denature. The mixture is then cooled, allowing the primers to hybridize to the complementary sequences of target DNA. Heat is then applied again to allow for the Taq polymerase to extend complementary strands from the primers. This process is then repeated hundreds or thousands of times, allowing for a large amount of amplification with ease. PCR has revolutionized the genetics research community, allowing millions of copies of DNA to be synthesized within hours. It is this efficiency that has made PCR become increasingly popular over the past thirty years. Another aspect of genetics that has revolutionized genetics research is gene synthesis. Gene synthesis is the process by which a physical sequence of DNA is constructed from a desired sequence in text format. Gene synthesis is carried out here, at Synbio Technologies, by our Syno® Platform. This allows us, at Synbio Technologies, to synthetically engineer genes up to 150 Kb in length. The sequence can then be amplified as many times as necessary by PCR. It is this connection between gene synthesis and PCR that has caused a large impact and various applications on the field of genetics.

The connection that these two technologies have is that gene synthesis uses PCR to amplify the gene of interest. The reliance is shown when PCR is used to amplify the synthetically engineered gene to the requested amount of copies. The gene of interest starts in text format, specified by the costumer, and put through our Syno Platform in order to generate the physical copy of the sequence. Throughout this process the possibility for errors within the sequence being generated can be quite high. For this reason, the Syno Platform includes multiple quality assessments to verify that the sequence of interest is identical to the one generated by Synbio Technologies. We offer one hundred percent sequence verification, analyzed with Sanger sequencing. After the sequenced is verified to be one hundred percent accurate, PCR is used to amplify the synthetically engineered gene. The resulting amount of product, specified by the customer, will then be shipped to your location, within as few as five days. The high quality product that the costumer receives from Synbio Technologies can then be put to use in various fields of genetics research that gene synthesis and PCR are commonly associated with. Gene synthesis itself offers a wide range of applications. It has been used to generate more effective vaccinations, as well as to study and improve the delivery of viral vectors used in gene therapy. Gene synthesis is the creation of the sequence of interest, but it is PCR that does the hard work to generate a sufficient amount of the sequence necessary to conduct these types of research. PCR offers the most efficient process to amplify the gene synthesis product in order to obtain a sufficient amount of product. Gene synthesis is a technology that is constantly utilized and relied upon, and few companies offer the efficiency and high quality product that Synbio Technologies offers.

This process, from costumer requested sequence to the final physical product, has been in use by Synbio Technologies for many years and we pride ourselves on our efficiency as well as high quality output. In addition to the efficiency and high quality output, we offer competitive prices and fast turnaround time. For gene synthesis, our prices starts at approximately $0.10 per base pair, which is near the lowest price in the gene synthesis industry. We offer the ability to synthesize a wide range of gene lengths, ranging from 100 base pairs to 150 Kb base pairs in length with one hundred percent accuracy. In addition to the range of lengths, using our Syno® 2.0 Platform, we are capable of generating many different structure of DNA. These structures include: repeated sequences, hairpin structures, high GC percentage, etc. These structures are of course then amplified by PCR in order to fulfill as many copies as the customer may need. After amplification by PCR and before shipping, the engineered sequences are then verified again in order to assure the quality of the product and the conservation of the requested product. This allows us to correct for any errors that may have occurred during the gene synthesis process or amplification by PCR. With the ability to generate large and complicated structures with this high of accuracy it is clear the Synbio Technologies is one of the leading companies in the gene synthesis industry.

Gene Synhtesis Related Services

  • Codon Optimization
  • Vector Construction
  • Small Genome Synthesis
  • Pathway Synthesis

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.