A relatively recent discovery, dating back to the early 1970s, in biology has revolutionized the field of genetics research. This discovery is known as gene synthesis, which allows for the manufacturing of a physical genetic sequence from a string of base pairs in text format. Gene synthesis allows for the creation of a genetic sequence that may not be preexisting within nature. Previously, a sequence of interest was required to be present within an organism in order to be extracted and later amplified to study its function. Gene synthesis allows us, at Synbio Technologies, to bypass this requirement entirely. This is accomplished through our, patent pending, Syno® Platform, which allows us to move from a sequence in text format to a physical copy with ease and accuracy. One common advantage that gene synthesis offers is the construction of gene synthesis vectors. Gene synthesis vectors vary widely in structure, function, length, and physical sequence characteristics. With the use of Synbio Technology’s Syno® Platform we are capable of not only synthesizing any gene of interest, up to 150 Kb in length, but constructing any gene synthesis vector specified by the customer. The generated gene synthesis vector can then be used in a wide range of applications, specific to each type of gene synthesis vector.
There are three main types of gene synthesis vectors that Synbio Technologies prides itself in its ability to generate with high accuracy in order to better suite the customer’s particular request. The three vectors are: short hairpin RNA (shRNA), transcription activator-line effector nuclease (TALEN), and CRISPR-Cas9. All three have similar functions, gene knockout also known as gene silencing, but have various methods in order to accomplish this. shRNA use RNA interference in order to block the transcription and translation of the gene/sequence of interest, rending it inactive. TALEN and CRISP-Cas9 use genome editing in order to render the gene/sequence of interest inactive. All three have been tested and proven as effective methods of gene silencing and inhibiting the resulting protein expression. Gene silencing is a common method within genetics research in order to determine the function of a gene. Once the gene is rendered inactive, researchers can study the resulting phenotype and infer on the function of gene of interest. Where gene synthesis comes into play is through our Syno® 2.0 and Syno® 3.0 Platforms Synbio Technologies is capable of constructing the requested sequence within any of these vectors, in addition to other popular vectors. This technology allows the customer to design a sequence, in text format, which allows Synbio Technologies to generate the requested gene synthesis vector. Once generated, the sequence and resulting vector will work to inhibit the endogenous gene, lowering protein expression and essentially silencing the gene of interest. As mentioned before, this silencing is achieved through the various methods of gene silencing utilized by each vector.
At Synbio Technologies we are extremely confident in our ability to construct these gene synthesis vectors to the customer’s exact specifications. We guarantee with one hundred percent accuracy within our generated sequences in order to produce the highest quality output. With this accuracy we can guarantee the best possible method of achieving a gene knockout through the use of our gene synthesis vectors. At Synbio Technologies we don’t only offer the highest quality product, but extremely fast turnaround times and competitive prices as well. Within as few as 10 days the customer’s gene synthesis vector will be constructed and be shipped to the customer specified location. With this confidence, high quality output, fast turnaround time, and competitive prices it is clear the Synbio Technologies is one of the leading companies within the gene synthesis industry.
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