Typically the genes with high GC content, hairpin structure and repetitive sequences present great challenges to standard gene synthesis process. Synbio Technologies’ new NGTM Codon system (patent pending) provides a robust and sophisticated algorithm to solve those challenges and enable successful synthesis of even the most complex sequences as demonstrated by the case studies below.
i. A gene with high GC content and repeats
This gene has a length of 1,606 bp with GC content: of 71.98% and 176 CpG. The gene was delivered in 5 weeks.
ii. A gene with low GC content (AT rich) and palindromic repeats
This gene has a length of 580 bp and GC content of 32.76% and palindromic repeats of 15 loxP sites. The gene was delivered in 4 weeks.
iii. Large yeast genome DNA assembly
This 49 kb long yeast genome fragment was made through one-step assembly in yeast.