Gene Synthesis Case Studies

Typically the genes with high GC content, hairpin structure and repetitive sequences present great challenges to standard gene synthesis process. Synbio Technologies’ new NGTM Codon system (patent pending) provides a robust and sophisticated algorithm to solve those challenges and enable successful synthesis of even the most complex sequences as demonstrated by the case studies below.

i. A gene with high GC content and repeats

This gene has a length of 1,606 bp with GC content: of 71.98% and 176 CpG. The gene was delivered in 5 weeks.

Figure 1. This graph shows the GC distribution and secondary structure of the mentioned gene.

ii. A gene with low GC content (AT rich) and palindromic repeats

This gene has a length of 580 bp and GC content of 32.76% and palindromic repeats of 15 loxP sites. The gene was delivered in 4 weeks.

Figure 2. This graph shows the GC distribution and secondary structure of the mentioned gene.

iii. Large yeast genome DNA assembly

This 49 kb long yeast genome fragment was made through one-step assembly in yeast.

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Figure 3. Illustration of the gene suquence assmbly and DNA gel eletrophoresis results for the successful synthesis of 49 kb yeast genome