A Brief Introduction of Hybridoma/Monoclonal Sequencing Technology

The global monoclonal antibodies market accounted for 85.4 billion USD in 2015 and is expected to exhibit a growth rate of 5.7% over the forecast period (GRAND VIEW RESEARCH) [1]. The rapid development of genomics research and next generation sequencing technology not only reduces the cost of monoclonal antibody research, but also promotes the research process. Monoclonal antibody drugs are expected to bring billions of dollars of business opportunities to pharmaceutical manufacturers within the forecast period.

Monoclonal antibody market by source type, 2013 – 2024 (USD Billion)

Hybridoma Sequencing Technology

Through the utilization of hybridoma sequencing technology, the variable regions of heavy chain and light chains of monoclonal antibodies can be obtained. This information will lay a solid foundation for the subsequent production of high affinity and high specificity antibodies by recombinant engineering antibody technology.

With the development of sequencing technology, there are several relatively mature methods to obtain antibody sequences, but each method has its own characteristics [2].

MethodsAdvantagesDisadvantages
High-throughput RNA-sequencing technologiesHigh accuracyHigh cost and long turnaround time
PCR and Sanger sequencingLow costDifficulties in designing universal primers and success rates of only 80-90%
5’ RACE (rapid amplification of 5’ cDNA ends)Simple, fast, and low costLow specificity and introduced mutation
de novo protein sequencing (mass spectrometry & X-ray)High accuracyHigh cost and long turnaround time

Hybridoma/Monoclonal Antibody Sequencing Service – Synbio Technologies

Synbio Technologies’s specialized hybridoma/monoclonal antibody sequencing technology can accurately obtain the sequence of multiple species, including rat, mouse, hamster, human, rabbit, etc. Based on our Syno® DNA Synthesis Platform and antibody produce platform, we can provide a one stop solution from hybridoma sequencing to recombinant antibody research.

Standard Hybridoma Sequencing Process

standard-hybridoma-sequencing-process

Related Services

References

[1] GRAND VIEW RESEARCH: https://www.grandviewresearch.com/industry-analysis/monoclonal-antibodies-market.
[2] Lena Meyer, Tomás López, Rafaela Espinosa, etc. A simplified workflow for monoclonal antibody sequencing. PLoS One. 2019; 14(6): e0218717.