The transfection of plasmids into mammalian cells directly incorporates genetic material into the genome to form a stable transfection. Stable transfection is used to create cell populations that will continue to express the genetic characteristics of the foreign material and then pass it on to the offspring. In order to achieve stable transfection, there should be selective pressure to force cells to integrate plasmid DNA into the genome.
Reporter genes are usually used to easily monitor the transfection efficiency and expression levels in cells. Synbio Technologies provides a variety of reporter gene expression vectors to meet the screening needs of different cell lines and efficiently screen expression according to cell-specific screening conditions.

Different promoters have different activation activities in different cells. For example, in hematopoietic stem cells, SFFV has much better activation activity than CMV. There are also differences in tissue-specific expression. AAT is highly expressed in liver tissues and weakly expressed in other tissues. hSyn is highly expressed in nerve cells and weakly expressed in other tissues. Synbio Technologies provide a variety of fluorescent proteins, promoters, and mammalian expression vectors with selectable markers to meet the needs of cell-specific screening.

Selectable markers in mammalian cells work similarly to that in bacteria.
A table of the most commonly used selectable markers are listed below:

The promoters of mammalian expression vectors also have different applicable objects.
The most commonly used promoters are listed below:

* For more information please contact us at quote@synbio-tech.com.