Introduction to molecular biology
Molecular biology is a subfield in biology that studies the various topics and molecular mechanisms within cells that are important for proper cellular function. These topics can include any cellular mechanism ranging from cellular metabolism to utilizing a molecular function in order to successfully accomplish gene synthesis. These topics and mechanisms have the ability to be altered slightly to accomplish a particular application of interest. These mechanisms within molecular biology, have been studied intently and applied to various fields of biological research and development. The applications within molecular biology that this article will introduce are gene expression and protein synthesis. These endogenous mechanisms are vital to the proper cellular function within molecular biology that can be altered to successfully conduct both gene synthesis and protein synthesis.
Molecular biology for gene synthesis
One important aspect of the gene synthesis process is that it is not limited to DNA replication of a preexisting DNA fragment. Gene synthesis is capable of in vitro artificial DNA sequence synthesis, which allows for the synthesis of de novo genetic sequences. Contrary to traditional methods of DNA amplification, gene synthesis does not require that the sequence of interest be preexisting within nature. This is accomplished by using refined chemical methods for gene synthesis without a template DNA chain. The resulting templates are then connected to form a gene fragment, by use of various molecular biology mechanisms. With the development of molecular biology, researchers have the ability to synthesize any gene of interest up to and including 150 Kb in length. The development of more efficient gene synthesis technologies has also allowed the gene synthesis process to become more time efficient, accurate and cost effective. These contributing factors has led gene synthesis to become an important technique within molecular biology.
Molecular biology for protein express
The aim of gene fragment synthesis is for the resulting synthesized gene to be inserted into plasmid vector. After this is accomplished, the recombinant plasmid transcripts are then inserted into cells, where the recombinant plasmid will be expressed. This allows for the expression of the newly synthesized gene of interest and its resulting protein. In order to obtain the highest quality product and strongest protein expression the recombinant protein must first be purified. The resulting purified protein can then be used in subsequent experiments, specific to our customer’s interests. These experiments may include research contributing to the better understanding of protein structure, function and activity of cells, as well as the diagnosis of the certain diseases. All of applications can provide scientific basis for treatment and drug development. For these reasons, optimizing the gene synthesis expression at the protein level is of upmost important to us, at Synbio Technologies.
Synbio Technologies offers comprehensive molecular biology services, designed to fit our customer’s specific needs. These molecular biology services include gene synthesis, PCR cloning, subcloning, site-directed mutagenesis, vector construction and other related projects. The services Synbio Technologies offers rely upon our Syno® 1.0 and Syno® 2.0 platforms. These platforms allow us to generate the highest quality gene synthesis product for a cost effective price. In addition to this, Synbio Technologies also offers the bacterial and yeast expression systems. These systems help optimize the resulting protein product from our synthesized sequences. The whole process is done with such ease and efficiency, that we are confident our high quality purified recombinant protein will arrive to your bench in as soon as 6 weeks.
Molecular Biology Related Services