Plasmid DNA Preparation | DNA Preparation Services

For years, Synbio Technologies has provided comprehensive plasmid DNA preparation services to our customers from research institutes to both biotechnology and biopharmaceutical industries. We have developed and perfected a manufacturing pipeline designed to meet the requirements of the Good Manufacturing Protocol (GMP). These requirements guarantee both in-process controls and final product quality control, to ensure that the highest quality product will be delivered to our customers.

All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<100EU/mg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

Competitive Advantages

Express and Guaranteed Plasmid Preparation Services: USA based manufacturing allows high quality products and fast delivery.
Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.
Low Endotoxin Level: Animal-derived materials free, <100EU/mg, on request.
Strict Quality Control: ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation.
Intellectual Property (IP) Protection: All our customers’ intellectual project related rights are fully respected and protected.

Experimental Procedure

Endo Free* DNA Prep (High Copy) Guarantee	Endo Free* DNA Prep (Low Copy) Guarantee	Price ($)	TAT (days)
200 ug	50-100 ug	Starting from $65	~~4-6~~ 2-4
1 mg	250 ug-0.5 mg	quote	~~4-6~~ 2-4
10 mg	2.5 mg-4 mg	quote	~~5-7~~ 3-5
100 mg	25 mg-40 mg	quote	~~6-10~~ 4-8
500 mg	125 mg-200 mg	quote	~~7-12~~ 5-10

Standard Plasmid Preparation Package	Research Grade	Transfection Grade
Endotoxin Level	NA	<100EU/mg*
Quantity	0.1 mg to gram level	1 mg to gram level
Turnaround time	Starting at 3 2 business days^Fast	Starting at 7 5 business days^Fast
Quality Control Methods	Restriction enzyme analysis	Basic quality control tests Sterility assurance Restriction enzyme analysis Sequence verification
Recommended Applications	Standard DNA manipulations including gene cloning, PCR template, DNA marker, DNA library. Transient protein expression and protein expression test DNA storage Genome editing tools Large-scale genome research	in vitro/in vivo transfection Large scale protein expression Viral Packaging Cell therapeutic material Pre-clinical trials in gene therapy or vaccination Long-term DNA storage
Standard Delivery Package Contains	Prepared plasmid DNA, Certificate of analysis (COA) and QC report

Synbio Technologies plasmid preparation service focuses on quality. We have strict quality control which can increase quality stability and reduce batch difference to satisfy customers’ high-quality needs.

Quality Control	Standard Range	Measurement Technique
Appearance	Clear, transparent	Visual inspection
A260/280 Analysis	1.80~2.00	UV spectrophotometer
Concentration	Up to 5mg/ml, default 1±0.05 mg/ml	UV spectrophotometer
Supercoil Percentage Analysis	>95%	Density scan on agarose gel
RNA Residue Analysis	Not detectable	Visual inspection on agarose gel
Genomic DNA Analysis	Not detectable	Visual inspection on agarose gel
Sterility Assurance	No clone growth on LB plate > 48h	Incubate 0.1mg of product on LB plate for >48h
Restriction Enzyme Analysis (optional)	As expected, no other minor band	Incubate with enzyme and analyze on agarose gel
Sequence Verification (optional)	Correct	DNA Sequencing
Endotoxin Analysis (optional)	<0.005EU/μg	Endotoxin Analysis Assay

Competitive Advantages

Experimental Procedure

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