Plasmid DNA preparation is a basic but very important step in scientific research. For years, Synbio Technologies has provided comprehensive plasmid DNA preparation services to our customers from research institutes to both biotechnology and biopharmaceutical industries.
Synbio Technologies conducts comprehensive quality control of our plasmid products to provide you with aseptic plasmids with no RNA pollution or genome pollution. All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (< 100EU/mg, <30EU/mg, <5EU/mg, on request). According to customer’s different application needs, standard plasmid DNA preparation services are divided into two levels, the research level and the transfection level. The plasmid DNA preparation process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.
Competitive Advantages
- Express and Guaranteed Plasmid Preparation Services: USA based manufacturing allows high quality products and fast delivery.
- Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.
- Low Endotoxin Level: Animal-derived materials free, <100EU/mg, on request.
- Strict Quality Control: ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation.
- Intellectual Property (IP) Protection: All our customers’ intellectual project related rights are fully respected and protected.
Experimental Procedure
| Quality Control | Standard Range | Measurement Technique |
| Appearance | Clear, transparent | Visual inspection |
| A260/280 Analysis | 1.80~2.00 | UV spectrophotometer |
| Concentration | Up to 5mg/ml, default 1±0.05 mg/ml | UV spectrophotometer |
| Supercoil Percentage Analysis | >95% | Density scan on agarose gel |
| RNA Residue Analysis | Not detectable | Visual inspection on agarose gel |
| Genomic DNA Analysis | Not detectable | Visual inspection on agarose gel |
| Sterility Assurance | No clone growth on LB plate > 48h | Incubate 0.1mg of product on LB plate for >48h |
| Restriction Enzyme Analysis (optional) | As expected, no other minor band | Incubate with enzyme and analyze on agarose gel |
| Sequence Verification (optional) | Correct | DNA Sequencing |
| Endotoxin Analysis (optional) | <0.005EU/μg | Endotoxin Analysis Assay |