Plasmid DNA Preparation | DNA Preparation Services

For years, Synbio Technologies has provided comprehensive plasmid DNA preparation services to our customers from research institutes and biotechnology and biopharmaceutical industries. We have developed and perfected a manufacturing pipeline designed to meet the requirements of the Good Manufacturing Protocol (GMP). These requirements guarantee both in-process controls and final product quality control, to ensure that the highest quality product will be generated. All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<0.005EU/μg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research. In addition to our services, we offer complete and detailed documentation that allows us to track and monitor the whole manufacturing process from start to finish. Throughout this process, our project management team will monitor the whole operation process to ensure on-time delivery. This process culminates with extensive quality control assays including sequence verification, restriction enzyme digestion, and endotoxin analysis. After passing our strict quality control assays, we guarantee the quantity and quality of our delivered products. The result is high quality plasmid DNA ready to ship to our customers all over the world. Additionally, protect your intellectual property, we are willing to sign an agreement or contract if deemed necessary. We guarantee to protect our customers’ benefits and their products. Dispensing is also available on your request. Additional expense may be charged depending on the quantity and other requirements.

Competitive Advantages

Express Plasmid Preparation Services: USA based manufacturing allows high quality products and fast turnaround time.
Low Endotoxin Level: Animal-derived materials free, <0.005EU/μg, on request
GMP-Like Manufacturing Pipeline: Complete and detailed manufacturing documentation
Strict Quality Control: ISO9001/2015 quality management
Fast procedure and on-time delivery

Experimental Procedure

Synbio Technologies plasmid preparation service focuses on quality. We have strict quality control which can increase quality stability and reduce batch difference to satisfy customers’ high-quality needs.

Quality Control	Standard Range	Measurement Technique
Appearance	Clear, transparent	Visual inspection
A260/280 Analysis	1.80~2.00	UV spectrophotometer
Concentration	Up to 5mg/ml, default 1±0.05 mg/ml	UV spectrophotometer
Supercoil Percentage Analysis	>95%	Density scan on agarose gel
RNA Residue Analysis	Not detectable	Visual inspection on agarose gel
Genomic DNA Analysis	Not detectable	Visual inspection on agarose gel
Sterility Assurance	No clone growth on LB plate > 48h	Incubate 0.1mg of product on LB plate for >48h
Restriction Enzyme Analysis (optional)	As expected, no other minor band	Incubate with enzyme and analyze on agarose gel
Sequence Verification (optional)	Correct	DNA Sequencing
Endotoxin Analysis (optional)	<0.005EU/μg	Endotoxin Analysis Assay

	Research Grade		Application Grade
	Express Plasmid Preparation^New	Standard Plasmid Preparation	Application Grade
Endotoxin Level	NA		<0.005EU/μg
Quantity	<200μg	0.1 mg to gram level	1 mg to gram level
Turnaround time	Starting at 1 business day	Starting at 3 business days	Starting at 7 business days
Quality Control Methods	Restriction enzyme analysis		Basic quality control tests Sterility assurance Restriction enzyme analysis Sequence verification
Recommended Applications	Standard DNA manipulations including gene cloning, PCR template, DNA marker, DNA library. Transient protein expression and protein expression test DNA storage Genome editing tools Large-scale genome research		in vitro/in vivo transfection Large scale protein expression Viral Packaging Cell therapeutic material Pre-clinical trials in gene therapy or vaccination Long-term DNA storage
Standard Delivery Package Contains	Prepared plasmid DNA, Certificate of analysis (COA) and QC report