For years, Synbio Technologies has provided comprehensive plasmid DNA preparation services to our customers from research institutes to both biotechnology and biopharmaceutical industries. We have developed and perfected a manufacturing pipeline designed to meet the requirements of the Good Manufacturing Protocol (GMP). These requirements guarantee both in-process controls and final product quality control, to ensure that the highest quality product will be delivered to our customers.
All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<0.1 EU/μg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.
Competitive Advantages
- Express and Guaranteed Plasmid Preparation Services: USA based manufacturing allows high quality products and fast delivery.
- Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.
- Low Endotoxin Level: Animal-derived materials free, <0.1 EU/μg, on request.
- Strict Quality Control: ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation.
- Intellectual Property (IP) Protection: All our customers’ intellectual project related rights are fully respected and protected.
Experimental Procedure
| Quality Control | Standard Range | Measurement Technique |
| Appearance | Clear, transparent | Visual inspection |
| A260/280 Analysis | 1.80~2.00 | UV spectrophotometer |
| Concentration | Up to 5mg/ml, default 1±0.05 mg/ml | UV spectrophotometer |
| Supercoil Percentage Analysis | >95% | Density scan on agarose gel |
| RNA Residue Analysis | Not detectable | Visual inspection on agarose gel |
| Genomic DNA Analysis | Not detectable | Visual inspection on agarose gel |
| Sterility Assurance | No clone growth on LB plate > 48h | Incubate 0.1mg of product on LB plate for >48h |
| Restriction Enzyme Analysis (optional) | As expected, no other minor band | Incubate with enzyme and analyze on agarose gel |
| Sequence Verification (optional) | Correct | DNA Sequencing |
| Endotoxin Analysis (optional) | <0.005EU/μg | Endotoxin Analysis Assay |