Probe Synthesis

Nuclei acid probes are sequences of single stranded DNA or RNA fragments that have the potential to bind specifically to their complementary nucleic acid sequences through a process called hybridization. Nuclei acid probes are usually labeled with a detectable moiety such as radioisotopes, epitopes, fluorophores or biotin to facilitate the detection of their respective target molecules. Recently, nuclei acid probes are becoming a routinely used tool in various research areas. This increased prevalence is due to the increased availability of nuclei acid sequences in databases and advances in molecular biology, such as PCR and NGS. A few examples of these advances include: molecular diagnosis, chip sequencing, human genome engineering, tumor research, forensic identification, precision medicine, IVD, etc.

With our propriety synthesis platform and professional research team, Synbio Technologies is able to produce high quality nucleic acid probes with batch consistency for both routine and specialized applications. Additionally, we can provide a wide variety of modifications or labeling at the 5′ and 3′ ends or a designated position of the probe according to the specific need of our customers.

Competitive Advantages

  • High Purity: Special purification processes ensure efficient removal of small molecule fluorescent residue and purity up to 98%.
  • Strict Quality Control: ISO9001/2015 quality control management system ensures real-time monitoring of probe production and batch to batch consistency.
  • Online Ordering System: Complete online primer/probe synthesis ordering system to streamline the ordering process.
  • Diverse Production Scale: Our experienced probe synthesis experts provide nm to uM scale through our optimized production processes.
  • Customizable Synthesis: Customized modulations and labeling is available upon request.

Service Specifications

TAMRA

BHQ1

BHQ2

MGB

DABCYL

The estimated turnaround time for research use is 3-5 days and industrial use is 1 – 2 weeks.

Service Standards for Research or Industrial Use

Case Studies

Purity is one of the main factors affecting the effectiveness of a nucleic acid probe. Synbio Technologies’ advanced and optimized purification techniques can maximize the elimination of remaining raw materials and other impurities from the target probe, as illustrated below.

Figure 1. The spectrum shows the peak of probe product purified with the conventional method. ☆ represents the target product and O represents impurities. The spectrum shows that impurities were not successfully separated from the target product.**
Figure 2.This spectrum shows the peak of probe product purified with Synbio Technologies’ optimized purification method. ☆ represents target product and O represents impurities. The spectrum shows that impurities were further separated from the target product.**
** Sample crude product

Figure 3. QC analysis of the target product purified with Synbio Technologies’ purification method. The MS (left) analysis shows the mass of the product was comply to its theoretical molecular weight without N± peak; the HPLC (middle) analysis shows the purity of the product was >90%; and the UV (right) shows the absorbance of the product was consistent with its corresponding parameters.