Insect System

Baculovirus insect cell system offers several unique advantages over E. coli expression system, such as post-translational modifications, higher yield for secreted protein, and proved solubility. It is appropriate for expressing proteins that maybe harmful to mammalian cells, such as kinases and toxic proteins. With years of experience in protein expression, Synbio Technologies possesses abundant experience in baculovirus Insect cell protein expression, particularly the expression of toxic proteins, kinases, large proteins, membrane protein and intracellular proteins.

Insect System Advantages

Proprietary NGTM Codon Optimization Technology: Significantly improve protein expression levels and success rate.
Multiple Purification Strategies: Affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc., providing diversified choice of protein purities.
Comprehensive Protein Identification Services: SDS-PAGE, Western Blot, MALDI-TOF, HPLC, etc.

Service Specifications

Service StepsDescriptionDeliverablesTurnaround TimePrice
Gene Synthesis & Subcloning (optional)
  • Gene synthesis and subclone to appropriate vector
  • Restriction enzyme cut and sequencing report
1-2 weeksQuote
Virus Generation
  • Generation of recombinant Bacmid DNA
  • Generation of P1 stock, P2 stock and determination of virus titer
  • Experiment report
3-5 weeks
Pilot Protein Expression and Purification
  • Pilot expression evaluation
  • Pilot purification and purity verification
  • Protein (50-500 ug)
  • Expression evaluation report
1-2 weeks
Protein Expression
  • 1 L insect cell expression
  • 1-step affinity purification
  • Purified protein
  • Certificate of analysis
1-2 weeks

* The delivery time of this step bay vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.