Metabolic Pathway Synthesis

Since the renaissance of synthetic biology in 2000, the research of high value-added natural product metabolic engineering combining with synthetic biology has become one of the most promising fields. In 2003, Jay Keasling’s team first reconstructed and accomplished the functional verification of the artemisinin biosynthetic pathway in E.coli. Over the next 10 years, Keasling’s team successfully streamlined the process of host selection, optimization, and industrialization. The synthesis and construction of metabolic pathways along with the necessary assembly techniques play a fundamental supporting role in this kind of research.

Research Strategy

design-and-optimization-of-metabolic-pathways
Design and Optimization of Metabolic Pathways
  • Confirm metabolic synthesis related enzymes of intermediates
  • Design metabolic pathways at all levels
  • Optimize expression level with NGTM Codon Technology
construction-and-transformation-of-pathway Modules
Construction and Transformation of Pathway Modules
  • Construction and assembly of modules on each pathway
  • Selected strains are transformed and synthesized
validation-and-analysis-of-metabolites
Validation and Analysis
of Metabolites
  • Separation validation and quality analysis of metabolites
  • Improvement and determination of metabolic pathways
scaled-and-industrialized-development
Scaled and Industrialized
Development
  • Research to achieve scaled production
  • Expand the potential applications of metabolic pathways

Service Advantages

  • One-Stop Service
  • Synbio Technologies has complete DNA Synthesis Platforms which provides one-stop synthetic biology solutions.

  • Codon Optimization
  • Synbio Technologies has independent intellectual NGTM Codon optimization software, through which customers can optimize sequences and design synthesis schemes for free.

  • Efficient Vector Construction
  • Synbio Technologies has a comprehensive functional component library, which can complete all construction and verification services with high throughput and has successfully constructed a vector up to 150 Kb.

  • Professional Assembly Technology
  • Synbio Technologies’ self-developed synthetic assembly technology provides customizable and cost-effective gene synthesis and assembly services. This technology is capable of assembling single DNA sequences up to 150 kbp.