CRISPR-Cas9 sgRNA Library
CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough in genome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that with TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. Synbio Technologies offers the designing, construction and validation of CRISPR-Cas9 gene target system through our patent pending platform in a highly cost and time-effective approach. We offer one stop solution for CRISPR Cas9 projects, including genome scale gene targeting.
Competitive Advantages of CRISPR-Cas9
- Applicable in any type of cell lines
- Simultaneously knockout multiple targets
- More efficient
- Fast turnaround time
Services Specifications
- CRISPR-Cas9-based primer design
- CRISPR-Cas9-based plasmid construction
- CRISPR-Cas9-based target locus validation
- CRISPR-Cas9-based stable cell line construction
- CRISPR-Cas9-based stem cell construction
- Certificate of analysis (COA) for project
- Lyophilized plasmid DNA (2~5 µg), or libraries
- Sequencing chromatogram
- QC report
Service Type | Price | Turnaround Time (Business Days) |
---|---|---|
CRISPR-Cas9-based primer design | Inquiry | |
CRISPR-Cas9-based plasmid construction | ||
CRISPR-Cas9-based target locus validation | Inquiry | |
CRISPR-Cas9-based stable cell line construction | ||
CRISPR-Cas9-based stem cell construction |
Applications
- CRISPR-Cas9-based gene knock-out
- CRISPR-Cas9-based gene knock-in
- CRISPR-Cas9-based transcription activation
- CRISPR-Cas9-based transcription inhibition