CRISPR-Cas9 is one of the most convenient types of gene editing technology, and has been widely used in editing genes of many species[1]. CRISPR-Cas9 generally involves construction a plasmid containing the specific endogenous promoter and connection of the Cas9 gene and a synthetic sgRNA, followed by transfer into the cell or animal. However, plasmid construction and verification are both tedious and time-consuming. The degradation of the plasmid is relatively slow, which may prove to be inconvenient for subsequent experiments.
To improve the efficiency of the CRISPR-Cas9 system, Synbio Technologies has developed ready-to-use sgRNA synthesis services. We can perform sgRNA target design, DNA template synthesis of sgRNA, sgRNA in vitro transcription, and sgRNA purification, to provide customers ready-to-use sgRNA that can be directly transfected into cells or animals. Synbio Technologies’s ready-to-use sgRNA saves time on plasmid construction, and avoids the drawbacks of potentially non-degraded plasmids.
According to the publications, in vitro transcription of sgRNA has successfully edited the genes of different species including zebrafish[2], mouse[3]as well as filamentous fungi[4]etc. Synbio Technologies has also designed 3 universal negative control sgRNA for Human and Rat genome: Syno®-negative controls sgRNA1, sgRNA2, and sgRNA3. These sequences are used as a negative control in Human and Rat gene/genome editing experiments.
Service Advantages:
- One-stop solution: Synbio Technologies provides integrated services from sgRNA target design to high purity ready-to-use sgRNA production.
- Fast delivery: In just 3 business days, Synbio Technologies will deliver up to 20μg of customized ready-to-use sgRNA
- Convenience: ready-to-use sgRNA can be directly injected into animals or transfected into cells, improving the efficiency of gene editing experiments
Case Study – Synbio Technologies:
Synbio Technologies has designed a number of sgRNAs to target several genes in mouse, and performing in vitro transfection. The experimental period was shortened to 2 days, and the sgRNA amount was increased to 10-20 μg. This change could means a significant jump in efficiency for synthetic biology experiments utilizing CRISPR-Cas9.
Workflow:

Result:
- Clone DNA template into pUC57 vector, the sequencing result (Fig. 1) coincided with the designed sequence.
- Agarose gel electrophoresis of sgRNA obtained by in vitro transcription, clear bands shown in Fig. 2.
- sgRNA verification: Transcript sgRNA into cDNA, design sgRNA amplification primer, and obtain the complementary DNA sequence by PCR reaction. Clone DNA sequence into pUC57 vector; sequencing result (Fig. 3) showed the sgRNA sequence is correct.
*The template of Lane 1 is reverse transcripted cDNA, The template of Lane 2 is sgRNA digested by DNase I; The template of Lane 3 is in vitro transcripted DNA
Service Specifications:
Service Name | Product/Service Specifications | Turnaround Time (business day) | Deliverables | Price |
Ready-to-use sgRNA synthesis | sgRNA design DNA template synthesis In vitro sgRNA transcription and purification | <10 sgRNA, 5 10-20 sgRNA, 10 >20 sgRNA,Inquiry | sgRNA COA | Inquiry |
Syno® negative control sgRNA | Negative control sgRNA In vitro sgRNA transcription and purification | 5days | Syno®-negative control sgRNA1 Syno®-negative control sgRNA2 Syno®-negative control sgRNA3 | Inquiry |
How to order
![]() Tel +1 732-230-3003 | ![]() quote@synbio-tech.com | ![]() Fax +1 609 228 5911 | ![]() Online Inquiry online inquiry submission form |
References:
[1]Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity [J]. Science. 2012,337(6096):816-821.
[2]Xiao A, Wang Z, Hu Y, et al. Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish [J]. Nucleic Acids Res. 2013,41(14):e141.
[3]Fujii W, Kawasaki K, Sugiura K, Naito K. Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease [J]. Nucleic Acids Res. 2013,41(20):e187.
[4]Liu R, Chen L, Jiang Y, Zhou Z, Zou G. Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Cell Discovery. 2015.7.