Metagenome Sequencing


What is metagenome sequencing?

Metagenome is the study of the genome of the entire microbial community in a specific environmental sample. This is achieved by directly extracting the DNA of an environmental sample for high-throughput sequencing without isolation and culture. Metagenomic sequencing promotes research focusing on the macroscopic characteristics of microbial community structure, community diversity, species classification, functional activity, mutual cooperation and their relationship with the environment. Using this approach scientists can analyze the genetic composition and function of environmental micro-population, interpret the diversity and abundance of micro-populations, and explore the relationship between microorganism and environment, microorganism and hosts. It can more accurately reflect the actual living conditions of microorganisms.

Metagenome sequencing does not depend on the isolation and culture of microorganisms. It overcomes the technical limitations of traditional pure culture methods, expands the utilization space of microbial resources and provides an effective tool for microbial research. Currently, metagenome sequencing has been widely used in environmental and microbial research.

Synbio Technologies’ metagenome sequencing service can detect whole genome DNA that was extracted from any starting source such as soil, air, water, silt, and intestinal flora etc. In addition to purified genomic DNA, we can also provide samples of soil, feces, fluids and tissues etc.

Synbio Technologies’ Advantages

  • Higher throughput and shorter turnover times
    High-throughput coverage can comprehensively detect the microorganism information in the ecological environment. Single sequencing can fully explore the microflora and functional genes in environmental samples for all species within the sample and deliver analysis reports in as few as 20 business days.
  • Higher efficiency and faster sequencing speed
    Metagenome sequencing utilizes an advanced HiSeq 4000 sequencing platform to quickly and efficiently generate high-quality sequencing data with longer read lengths.
  • Higher accuracy and more detailed information
    Synbio Technologies can provide high accuracy, reliable quality products as well as detect single base differences and find high or low abundance species genes.
  • Experienced and professional
    We provide multiple primers and scientific experimental design, which can detect samples with high DNA content of eukaryotes and meet various testing requirements. We also provide comprehensive antibiotic factor analysis and provide customized analysis services according to our customers’ needs.

Standard Metagenome Sequencing Process

metagenome-sequencing

Service Specifications

Service ItemsSample TypeSequencing PlatformSample Standard
metagenome sequencingmetagenome sampleHiSeq 4000, PE150Environmental samples: Soil, 5-10g; Faeces, 3-5g; Sediment: 5-10g; Liquid: 2ml.
Water body: filter with 0.22μm or 0.45μm filter membrane, Water sample volume, 5-10mL, optimum membrane area 3-4cm. DNA: the total ≥ 1 μg、concentration ≥ 10 ng/μL、1.8 < OD260/280< 2.0

How to order

If you have any questions, please feel free to contact us for assistance in during business hours. Our experienced project managers will provide you with professional support to ensure the success of your project.

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