Single B Cell Antibody Sequencing Analysis


single-b-cell-antibody-sequencing-analysis

Single B cell antibody technologies offer novel mechanisms for the discovery of naturally occurring antibody drugs. This is accomplished by combining high throughput techniques with low sifting rates and avoiding drawbacks of unnatural pairing from in vitro screening. Synbio Technologies provides single B cell antibody services including the development and preparation of B cell antibodies, B cell antibody sequencing and deep analysis, etc.

B cells, also known as B lymphocytes, mainly in charge of humoral immunity of human bodies and present function under antigenic stimulation. When an adaptive immune response is warranted, activated B cells proliferate and undergo somatic hypermutation of their B cell receptor (BCR) to form diverse cell clonings. Additionally, as a complex molecule, antibodies have three complementarity determining regions (CDRs) in each heavy (VH) and light (VL) chains. Moreover, the CDR3 of the heavy chain is most abundantly altered and tends to perform at a high level of hypermutation at the junction between the V, D and J gene regions. Therefore, the natural VH/VL antibodies produced by B cells are critical for understanding antibody biology and design.

Synbio Technologies’ GenoTM Ab Platform provides professional single B-cell antibody sequencing analysis services to our customers. These services aid our customers to better assess the diversity of their immune system of interest as well as the relationship between the generated antibody libraries and diseases. Both aspects have large implications in the field of antibody research and development.

Featured Advantages

  • Internal antibody database platform to score the antibody’s heavy and light chain pairs to screen the most logical intact antibody sequence
  • Unique in-house data mining tools to pre-process antibody sequencing to minimize sequencing errors such as false positives
  • Experienced research team with complete bioinformatics analysis technology to mine high-value antibody information

Service Process

single-b-cell-antibody-sequencing-analysis-1


Synbio Technologies Case Study

Synbio Technologies’ Bioinformatics team analyzed various antibody chrematistics based on the client’s original antibody sequencing data. These characteristics included: the VDJ recombination, V/J gene frequency, frequencies of V-J pairing, CDR3 length analysis, B cell clone count, receptor diversity analysis, intergroup diversity difference analysis, cloning and expression of CDR3 lengths. The final professional research reports were provided.

Results:
1) Frequency of VDJ gene usage analysis

Table1. the Frequency statistics of V gene usage in BCR samples

Gene_nameReadsFrequency
4-39*01279804.97
4-34*01124882.22
1-2*0250070.889
3-74*0147980.852
1-18*0130110.535
3-7*0128620.508
2-70*1323130.411
3-74*0322400.398
1-69*0119430.345
4-31*0315830.281

germline

2) Analysis of CDR3 region length

cdr3-length

3) Analysis of CDR3 kinds

Table2. Statistics of CDR3 amino acid sequence length by BCR sequencing

CDR3ReadsFrequencylen
DNNNDMR35600.0048697
VISRTL16880.0023086
AVDSNYQLI8990.0012299
AVNRDDKII7180.0009829
AATDSWGKLQ6960.00095210
AVTDSWGKLQ6270.00085710
AVNTGNQFY5950.0008149
AVTGGGNKLT5730.00078410
AVPTGTASKLT5620.00076911
LVGDTGRRALT5400.00073811
AEITLT5170.0007076

4) Ordering of total length sequence

Table3. Reads of BCR amino acid sequence total length

Amino Acid Gene LengthAmino Acid LengthAbundance
14911470.096324
201390.044324
5401430.040395
5291450.041238
5161470.041955
5041430.042773
4961410.043976
4411450.040898
4331450.041868
3871480.039055

5) Frequency analysis of VH gene difference between different samples

vh

6) Saturation analysis of sequencing

saturation-analysis-of-sequencingsaturation-analysis-of-sequencing-1

7) Diversity analysis of cloning

diversity-analysis-of-cloning

TCR/BCRShannon EntropyInverse Simpson
BCR19.7458340.758

Service specification

ServiceDetailsAnalysis SoftwareTAT
(Business Days)
Deliverables
Single B cell Antibody Sequencing AnalysisData mining of sequencing,
Antibody information analysis, etc.
R, Python
Software etc.
7~10Analysis Report

* Relevant services: Monoclonal Antibody Sequencing, Immune Repertoire Sequencing.

How to Order Single B Cell Antibody Sequencing Analysis Service

If you have any questions, please feel free to contact us for assistance during business hours. Our experienced project managers will provide you with professional support to ensure the success of your project.

online-quotation
Online Inquiry
email
quote@synbio-tech.com
call
+1 732-230-3003
fax
+1 609 228 5911