Vector Construction

Vector construction

Based on our Syno®2.0 and Syno®3.0 synthesis platforms, Synbio Technologies will deliver any synthetic vector with high accuracy and economical price, especially those for research in shRNA, TALENT and CRISPR-Cas9 projects.

1. shRNA Construction and Validation

RNA interference blocks transcription and translation and inhibits gene expression by introducing double stranded RNA. RNAi is a quick,convenient and effective method which is widely used to down-regulate gene expression. Using shRNA to silence a target gene of choice, RNAi is immensely useful in disease studies of viruses, tumors and genetic disorders.

Synbio Technologies can offer you the following services:

  • Cell-based shRNA services: including shRNA design, shRNA construction, shRNA transfection and function characterization
  • Lentivirus-based shRNA services: including vector construction, lentivirus particle packaging, RNAi validation and stable cell lines construction
  • Adenovirus-based shRNA services: including vector construction, adenovirus particle packaging and RNAi validation
  • Stem cell-based shRNA services
  • RNAi effects in stem cells

Services Specifications

ServicesPriceTAT (Business days)
shRNA vector constructionInquiry10
Cell-based shRNA servicesInquiryInquiry
Lentivirus-based shRNA servicesInquiryInquiry
Adenovirus-based shRNA servicesInquiryInquiry
Stem cell-based shRNA servicesInquiryInquiry

2.  TALEN Construction and Validation

TALEN (transcription activator-like effector nuclease) is a molecular tool frequently used for genome editing. It cuts target genes precisely, leading to highly accurate gene editing. The TALEN system provides an easier, simpler, and more affordable method of gene silencing than zinc finger nuclease (ZFN) technology, as the TAL effector protein can specifically bind to most nucleic sequences while ZFN is more limited in its recognition and is often affected by adjacent downstream and upstream sequences.

Synbio Technologies provides the following services:

  • TALEN plasmid construction
  • Downstream function validation
  • Construction of stable cell lines
  • Stem cell construction.

Services Specifications

ServicesPriceTAT (Business days)
TALEN-based plasmid constructionInquiry10
TALEN-based target locus validationInquiryInquiry
TALEN-based stable cell lines constructionInquiryInquiry
TALEN-based stem cell constructionInquiryInquiry

3. CRISPR-Cas9 Construction and Validation

CRISPR-Cas9 gRNA (CRISPR-Cas9 RNA-Guided nuclease) is the most recent breakthrough in genome editing technology after TALEN. It precisely edits DNA through the RNA-directed nuclease Cas9. Previous studies indicate that the on-target gene knockout rates were higher than that of TALEN and ZFN. The construction of Cas9 is also easier and more convenient. The CRISPR-Cas9 system has been widely applied in animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. Synbio Technologies offers construction and validation of the CRISPR-Cas9 system through our patent pending Syno®2.0 platform in a highly efficient and cost-effective way.

Applications

  • CRISPR-Cas9-based gene knock-out
  • CRISPR-Cas9-based gene knock-in
  • CRISPR-Cas9-based transcription activation
  • CRISPR-Cas9-based transcription inhibition

Synbio Technologies offers you the following services:

  • CRISPR-Cas9-based sgRNA design
  • CRISPR-Cas9-based plasmid construction
  • CRISPR-Cas9-based target locus validation
  • CRISPR-Cas9-based stable cell line construction
  • CRISPR-Cas9-based stem cell construction

Services Specifications

Service typePriceTAT (Business days)
CRISPR-Cas9-based primer designInquiry1-3
CRISPR-Cas9-based plasmid constructionInquiry10
CRISPR-Cas9-based target locus validationInquiryInquiry
CRISPR-Cas9-based stable cell line constructionInquiryInquiry
CRISPR-Cas9-based stem cell constructionInquiryInquiry

Delivery Form

Synbio Tech.’s standard package including:

  • Lyophilized plasmid DNA (4µg)
  • Sequencing chromatogram
  • Certificate of analysis (COA)
  • Virus particle (Optional)
  • Cell line (Optional)

How to order vector construction services

If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.


online-quotation
Online Inquiry
email
quote@synbio-tech.com
call
+1 732-230-3003
fax
+1 609 228 5911