Metabolic Flux Analysis

Metabolomics reflects static metabolite abundance, and the increase of a single metabolite may be due to either the activation of the synthetic pathway or the inhibition of the consumption pathway. Therefore, Metabolomics is often not enough to explain all the problems in the study of specific metabolic pathways and metabolic networks. As a complementary tool to metabolomics, Metabolic Flux Analysis (MFA) uses stable isotopes (13C or 15N) to label specific molecules and track their metabolic processes in an organism to obtain dynamic information about metabolites in metabolic pathways. MFA is the only tool that can quantify energy flows (i.e., ATP, cofactor generation and consumption) and their balances.


  • Medical Research: Tumor metabolism mechanism, immune related diseases, metabolic diseases, stem cell development and differentiation, etc.
  • Agriculture and Forestry: Simulate physiological processes of biology, bacterial metabolites, stress resistance, commercial crop quality, breeding, animal nutrition, meat quality, etc.

Competitive Advantages

  • Advanced Instrument Platform: Orbitrap LC-MS & UHPLC-QQQ-MS & GC-Q-MS.
  • Personalized Service: Professional bioinformatics teams & personalized bioinformatics analysis services.

MFA Work Flow


Service Specifications

Service TypesSample RequirementsPlatformPriceTurnaround TimeDeliverables
Glycolysis Flux Ratio
  • Cell≥4×106
  • Stored in liquid nitrogen or -80℃
  • Transportation by dry ice
  • Orbitrap LC-MS
  • GC-Q-MS
  • Raw data
  • Data analysis report
TCA Pathways Flux
Pentose Phosphate Pathways

Analysis Item

NumberAnalysis ItemNumberAnalysis Item
1Raw data preprocessing513C flux ratio differential analysis
2Isotopic distribution of metabolites6Hierarchical cluster analysis
3Identification of metabolic pathways7PCA analysis
413C flux ratio analysis

Data Analysis

Isotopic Information of Metabolites

Hierarchical Cluster Analysis

13C Flux Ratio Differential Analysis

PCA Analysis