Metabolomics

Following the ideas of genomics and proteomics, metabolomics is an emerging omics that is the qualitative and quantitative analysis of all metabolites in an organism to find the relationship between metabolites and physiological changes. Metabolomics is the study of endogenous small molecules with a relative molecular mass of less than 1500 Da, which analyzes the changes of metabolites of biological systems (cells, tissues, or organisms) after stimulation or disturbance.

Service Specifications

Untargeted metabolomics refers to the non-biased detection of the dynamic changes of all small molecule metabolites in cells, tissues, organs, or organisms after stimulation or disturbance by LC-MS, GC-MS, and NMR technology. Differential metabolites are screened by bioinformatics analysis, and then pathway analysis is performed to reveal the physiological mechanism of their changes.

Applications
  • Phenotypic and Physiological Functions
  • Pathological Study of Diseases
  • Clinical Diagnosis and Treatment
  • Drug Research
  • Food Science and Nutrition
  • Animal Husbandry and Agroforestry
  • Environmental Toxicology
Competitive Advantages
  • Advanced Instrument Platform: UHPLC-QE-MS & UHPLC-QTOF-MS & GC-Q-MS.
  • Abundant Commercial Databases: Many commercial databases, more than 2000 standard databases, more accurate qualitative data.
  • Strict Quality Control: Rigorous method validation in analytical processes.
  • Personalized Service: Professional bioinformatics teams, Personalized bioinformatics analysis services.
Untargeted Metabolomics Work Flow

Service Specifications
Service TypesSample TypeSample RequirementsPlatformPriceTurnaround TimeDeliverables
Untargeted Metabolomics(LC-MS)Cell≥107Thermo Q Exactive FocusInquireInquire
  • Raw data
  • Data analysis report
TissueAnimal tissue ≥100mg, plant tissue ≥1000mg, microorganism ≥60mg
Feces≥150mg
Untargeted Metabolomics(GC-MS)Bodily FluidSerum/plasma ≥100µL, urine ≥125µL, saliva ≥0.5mlGC-Q-MS
Exosome≥200µL
Soil≥5g

Project Design
Untargeted metabolomics is used to detect and quantify total metabolites in biological samples. The design idea of untargeted metabolomics experiments is to compare differential metabolites under different treatments or physiological states and to explore the difference of mechanisms. At least 8 biological replicates are required for cell, plant, and microbial samples in each group. At least 10 biological replicates are required for animal samples in each group. At least 30 biological replicates are required for clinical samples each group.

Analysis Item
NumberAnalysis ItemNumberAnalysis Item
1Raw data preprocessing6OPLS-DA analysis
2Data quality control7Differential metabolites expression analysis
3Base peak chromatogram8Cluster analysis of differential metabolites
4PCA analysis9ROC analysis
5PLS-DA analysis10KEGG pathway analysis

Data Analysis

BPC of Typical Samples

PLS-DA Score Plot

Correlation Heatmap of Differential Metabolites

PCA Score Plot

PLS-DA Permutation Test Plot

Pathway Impact Plot

Targeted metabolomics refers to several target compounds or target metabolites involved in a certain pathway. The targeted metabolomics method with high specificity, high sensitivity, and good reproducibility was established by using the standard compounds for absolute quantitative analysis of the target metabolites. Technique platforms include liquid chromatograph-mass spectrometer (LC-MS) and Selective Reaction Monitor / Multi Reaction Monitor (SRM/MRM).

Targeted Metabolites List
Metabolites CategoryMetabolites TypeMetabolites Name
Short-chain fatty acids7Acetic acid, Propionic acid, Butyric acid, Isobutyric acid, Pentanoic acid, Isovaleric acid, Hexanoic acid
TMAO and related metabolites5Trimethylamine N-oxide, Choline, Betaine, Creatinine, L-Carnitine
Amino acid22L-Asparagine, L-Phenylalanine, L-Valine, L-Lysine, L-Alanine, L-Serine, Glycine, etc.
Fatty acid51Methyl Caproate, Methyl Caprylate, Methyl Caprate, Methyl Undecanoate, Methyl Laurate, etc.
Neurotransmitter23Histamine, Tyramine, Levodopa, Tryptamine, et al
Bile acid40Allolithocholic acid, Lithocholic acid, Isolithocholic acid, Deoxycholic acid, Cholic acid, etc.

Applications
  • Short-Chain Fatty Acids: Regulating blood sugars, anti-inflammatory, improving intestinal functions, etc.
  • TMAO and Related Metabolites: Aquatic products, mammals, plants, cardiovascular diseases, diabetes, etc.
  • Amino Acids: Cardiovascular diseases, diabetes, plant stress tolerance, plant growth and development, etc.
  • Fatty Acids: Animal and plant oil, regulating cell membrane composition, etc.
  • Neurotransmitters: Tissue fluid and blood samples, particular chemical that acts as a messenger, etc.
  • Bile Acids: Liver and gall diseases, gastrointestinal diseases etc.
Competitive Advantages
  • Advanced Mass Spectrometry Platform: High sensitivity.
  • Strict Quality Control (QC): Set QC sample, standard curve R2>0.99.
  • Complete Data Analysis: Comprehensive targeted metabolic data analysis.
  • Targeted Metabolomics Work Flow Service Specifications
    Service TypesSample TypesPlatformPriceTurnaround TimeDeliverables
    Short-chain fatty acidsCell, Tissue: Animal tissue/plant tissue/microorganism, Feces Bodily Fluid: Serum/plasma/urine/saliva, Exosome, Soil, etc.GC-Q-MSInquireInquire
    • Raw data
    • Data analysis report
    TMAO and related metabolitesLC-MS/MS
    Amino acidsLC-MS/MS
    Fatty acidsGC-Q-MS
    NeurotransmittersLC-MS/MS
    Bile acidsLC-MS/MS

    Project Design
    Targeted metabolomics analysis has several characteristics, such as high specificity, high sensitivity, and quantitative accuracy. It can be combined with other experimental data to reveal relevant molecular mechanisms by accurate quantitative and qualitative analysis of a specific metabolite. On the other hand, it can also provide strong supports for the development and utilization of metabolic biomarkers in the future. Targeted metabolomics can be used to test hypotheses proposed by untargeted metabolomics experiments.

    Analysis Item
    NumberAnalysis ItemNumberAnalysis Item
    1Raw data preprocessing6OPLS-DA analysis
    2Standard curve drawing7Quantitative analysis of target metabolites
    3Total ion chromatogram (TIC)8Cluster analysis of target metabolites
    4PCA analysis9ROC analysis
    5PLS-DA analysis10KEGG pathway analysis

    Data Analysis

    TIC of Samples

    Metabolites Cluster Heatmap

    Z-score Plot

    Standard Curve

    Box Plot of Target Metabolites

    Metabolic Pathway Plot