Synthetic DNA Libraries

Synbio Technologies offers high quality DNA libraries of any size and complexity with an efficient time frame and at industry’s most competitive rate. Our synthetic DNA libraries are designed by an experienced bioinformatics team to facilitate various research applications, including functional genetics, directed evolution, protein engineering and antibody studies.

Why us?

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Proprietary synthesis platforms guarantee the successfully synthesis of multiple libraries and reasonable library preparation.

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High quality libraries can be accurately synthesized with large capacity (up to 1014 variants), coverage rate>95%, uniformity index<10.

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Flexible deliverables: oligo pools, PCR fragments, pooled clones or error-free separated colonies.

Library Types

Library TypeDescriptionSchema
Alanine Scan LibraryResearch on amino acid residues critical to protein function, interaction and shape by replacing an individual amino acid, with alanine, at every position.
Site Saturation LibrarySubstitute all 20 possible amino acids at any site to evaluate structure-function relationships and to generate improved protein variants.
Random Substitution LibrarySubstitute bases with triplet, random or degenerate ones to identify locations for specific analysis.
Antibody LibraryCreate a high diversity synthetic antibody library or improve existing antibody functionality such as specificity, immunogenicity, affinity, expression and/or aggregation.
Modular Substitution LibraryBuild new genetic modules, regions or pathways from DNA parts including regulatory elements and/or coding sequences.

Service Specifications

Length (nt/bp)Complexity AnalysisDiversity Analysis by SequencingDelivery Time
(Business Days)
Price
Site Saturation Library: NNK Library/ Trimer Library20-40Quote
Random Substitution Library20-40Quote
Modular Substitution Library20-40Quote

Library Deliverables

10 μg linear random PCR product library (up to 1011 randomly mutated molecules) OR 50-1000 ug plasmid.

Related Services

Next Generation Gene Synthesis

High throughput and most cost-effective.
DNA synthesis platform, starts at $0.09/bp for synthesis.

Vector Construction

shRNA, TALEN, CRISPR-Cas9 vector construction and validation.

DNA Sequencing & Gene Analysis

High quality standard present reliable gene sequencing and analysis services.

Protein Expression & Purification

Guaranteed 3 mg protein of 85% purity with our Syno® Gene to Protein Package in E. coli expression system.