Standard Oligo Synthesis

Synbio Technologies’ oligo synthesis platform equips state-of-the-art synthesizers and leading professional teams experienced in the oligo synthesis as well as modification technologies. This allows us to provide high-quality oligo products to scientific researchers and industrial customers around the world. By leveraging our advanced and innovative manufacturing process, Synbio Technologies offers a wide range of oligo synthesis options including standard oligos, degenerate primers, long oligos, ultra-high purity oligos synthesis, etc. In addition, we also provide high-throughput oligo synthesis to customers with large-scale oligo projects. Synbio Technologies is always competent to offer tailor-made approached to accomplish every request to oligo synthesis.

Competitive Advantages

  • High-Quality: We can achieve extremely low mutation and low error rates, in accordance with the stringent quality control standards for oligo synthesis.
  • Highly-Customizable: Flexible synthesis scales are available; four alternative purification options include DSL, HPLC, PAGE Plus, and PAGE.
  • Technical Support: Professional teams experienced in oligo synthesis and various modifications offer support throughout the production progress.
  • Cost Effective: Competitive prices and affordable services.

Service Specifications

Synbio Technologies provides a fast and convenient standard DNA oligo synthesis. Large scale and bulk custom DNA oligos can be ordered with the same high purity, competitive prices, and fast turnaround.

Standard Oligo Synthesis (Price per Base)
Synthesis Scale10nm25nm50 nmol100 nmol200 nmol1 µmol
11-59nt$0.07$0.10$0.15$0.30$0.40$1.00
60-90nt$0.08$0.12$0.18$0.35$0.50$1.20

* Longer oligos, 96/384 plate batch oligos or other large scale synthesis are available, please contact us for a quote.
* Please refer to the purification table for the final output corresponding to different synthesis scale.

Purification (Price per Oligo)
11-59ntPurification MethodSynthesis Scale10nm25nm50 nmol100 nmol200 nmol1 µmol
DSLFinal Yield1 OD2 OD5 OD10 OD15 OD50 OD
PriceFreeFreeFreeFreeFreeFree
PAGE PlusFinal Yield2 OD5 OD10 OD30 OD
Price$5.00$5.00$5.00$5.00
PAGEFinal Yield2 OD5 OD10 OD
Price$15.00$15.00$30.00
HPLCFinal Yield2 OD5 OD10 OD
Price$10.00$10.00$20.00
60-90ntDSLFinal Yield1 OD2 OD5 OD10 OD15 OD50 OD
PriceFreeFreeFreeFreeFreeFree
PAGE PlusFinal Yield2 OD5 OD10 OD30 OD
Price$5.00$5.00$5.00$5.00
PAGEFinal Yield2 OD5 OD10 OD
Price$15.00$15.00$30.00
HPLCFinal Yield2 OD5 OD10 OD
Price$10.00$10.00$20.00

* The total price of oligo=price/base*nt + purification price.
* If you need other synthesis scale and lengths, please contact us for a quote.
* For modified oligos please click Oligo Modification & Labeling

Genetic codes have the tendency to degenerate, which lead to the use of degenerate primers in PCR experiments to exhibit specific genes. Synbio Technologies provides both the design and synthesis of these degenerate primers, as well as custom degenerate oligo pool synthesis upon request.

  • Standard Service: No design fee, suitable for more than 95% PCR projects.
  • Superior Service: Utilized for certain projects sensitive to codon bias. Synbio Technologies can significantly reduce the deviation inherent in the synthesis procedures and realize the accurate proportional control.

SymbolBase TypeStandard RatioPrice
Standard LevelSuperior Level
RA,G50% : 50%FreeInquire
YC,T50% : 50%
MA,C50% : 50%
KG,T50% : 50%
SC,G50% : 50%
WA,T50% : 50%
HA,C,T33% : 33% : 33%
BC,G,T33% : 33% : 33%
VA,C,G33% : 33% : 33%
DA,G,T33% : 33% : 33%
NA,C,G,T25% : 25% : 25% : 25%

* Non-standard base proportion should be declared clearly, for example, N(20%A:30%C:40%G:10%T).

Based on the solid-phase oligo chemical synthesis technology, as the length of the synthetic sequence increasing, the undesired bands of N-1 type impurities are more unavoidable in the electrophoresis. However, some experiments still need to use long chain genes or ultra-long oligos (130-150 mer). In doing so, the synthesis efficiency and purification are pretty crucial.

Synbio Technologies’ Oligo Synthesis Platform utilizes an optimized ultra-long chain design and synthesis program to meticulously monitor and control the synthesis process. This process allows us to generate long high-quality oligos (90-129 mer) and even ultra-long oligos (130-150 mer) according to individual requests.

Length
QuantityPurification
Turnaround Time
Price
90-109 mer
1 ODPAGE / Dual-PAGE
5-6 business days
Inquire
110-129 mer
1 OD
5-7 business days
130-150 mer
0.5 OD
5-8 business days

Standard deliverables

Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and 384-well plate
Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.