Oligo Modification & Labeling

Synbio Technologies provides comprehensive modifications and labeling services to meet our customers’ specified research requirements. Our industry leading professional team utilizes state-of-the-art synthesizers to achieve both oligo synthesis and modifications. Our stringent quality control guidelines ensure the synthesis of high-quality modified oligos and that they are subsequently delivered in a timely manner. We can satisfy various research needs in biology, diagnostics, personal medicine, drug discovery, etc.

Synbio Technologies’ custom DNA oligos are designed and synthesized with rigorous quality control to ensure excellent overall quality while meeting our customers’ every request. Our specialized services include oligonucleotides modified with special bases, dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, digoxin, etc. Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’ or designated sites specified by the customer. This includes FAM, HEX, ROX, BHQ, Texas Red, and much more. The resulting oligos can be used in a variety of applications, including PCR, cloning, sequence verification, and gene detection.

Competitive Advantages

  • Comprehensive Modifications and Labeling:
    Large-scale sequencing verifying the average mutation rate to be lower than 1/1000
  • Stringent ISO9001 & ISO13485 Certified Processing for oligo synthesis, meticulously monitored and advanced traceability
  • Multiple Purification Options:
    Include desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
  • Technical Support:
    Professional teams experienced in oligo synthesis and various modifications offer support throughout the production progress

Services Specifications

Synbio Technologies provides a series of general modifications with the high purity and competitive prices. We accomplish this by focusing on the requests of modification with special chemical classifications. Browse general modifications listed below:

ClassificationModifications
Purification
(10-45bp)
(46-80bp)
Modified Bases
dU
HPLCPAGE&RP-HPLC
dI
5-Methyl dC
DBCO
5’ DBCO
HPLCRP-HPLC
3’ DBCO
PAGE&RP-HPLC
dT-DBCO
AMCA
5’ AMCA
HPLC
RP-HPLC
3’ AMCA
PAGE&RP-HPLC
dT-AMCA
Phosphorothioates
SPO3
HPLC
PAGE&RP-HPLC
Amino Linkers
5’Amino C6
HPLCPAGE&RP-HPLC
dT-Amino C6
3’Amino C7
5’Amino C12
RP-HPLC
Biotin
5’Biotin
HPLC
RP-HPLC
3’Biotin
PAGE&RP-HPLC
dT-Biotin
Digoxigenin
5’Digoxigenin
HPLC
RP-HPLC
3’Digoxigenin
PAGE&RP-HPLC
dT-Digoxigenin
Phosphorylation
5’ PO4
HPLCPAGE&RP-HPLC
3’ PO4
Hydrosulphonyl
5’ HS-C6
HPLC
RP-HPLC
3’ HS-C3
PAGE&RP-HPLC
Locked Nuleic Acid
LNA (A/G/C/U)
HPLC
PAGE&RP-HPLC
Fluoro Bases
2’-F-2’-dA
HPLCPAGE&RP-HPLC
2’-F-2’-dC
2’-F-2’-dG
2’-F-2’-dU
Spacer
dSpacer
HPLCPAGE&RP-HPLC
Spacer C3
Spacer C9
Spacer C18

Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ and Texas Red, etc.

Dye/Quenchers3′-5′-Designated SitesPurification
(10-45bp)
(46-80bp)
FAM
RP-HPLCRP-HPLC/Dual RP-HPLC
TET
JOE
HEX
SIMA
TAMRA
ROX
Cy5
Cy3
Quasar670
Quasar570
DyLight547
RP-HPLC
DyLight647
Texas Red
BHQ-1
Dual RP-HPLC
BHQ-2
BHQ-3
Eclipse
DABCYL

Despite of single fluorescent group, Synbio Technologies also offers dual/multiple labeling services including TAMRA, BHQ, DABYCL, ELCISPE, etc. The most common Dyes and Quenchers are listed below:

DyesQuenchersPurification
(10-45bp)
(45-80bp)
5’6-FAM
3’TAMRA
Dual RP-HPLC
RP-HPLC & IE-HPLC
3’BHQ-1
RP-HPLC & IE-HPLC
3’Eclipse
3’DABCYL
5’TET
3’TAMRA
Dual RP-HPLC
RP-HPLC & IE-HPLC
3’BHQ-2
RP-HPLC & IE-HPLC
3’DABCYL
5’HEX
3’TAMRA
Dual RP-HPLC
RP-HPLC & IE-HPLC
3’BHQ-1
RP-HPLC & IE-HPLC
3’BHQ-2
3’DABCYL
5’TAMRA
3’Eclipse
RP-HPLC & IE-HPLC
3’BHQ-1
3’BHQ-2
3’DABCYL
5’ROX
3’Eclipse
RP-HPLC & IE-HPLC
3’BHQ-1
3’BHQ-2
3’DABCYL
5’CY5
3’BHQ-1
RP-HPLC & IE-HPLC
3’BHQ-2
3’BHQ-3
3’DABCYL
5’CY3
3’BHQ-2
RP-HPLC & IE-HPLC
5’JOE
3’Eclipse
RP-HPLC & IE-HPLCRP-HPLC & IE-HPLC
3’BHQ-1
3’BHQ-2
3’DABCYL
3’TAMRA
Dual RP-HPLC
5’Texas Red
3’BHQ-2
RP-HPLC & IE-HPLC

Standard Deliverables

  1. Lyophilized dry DNA oligo powder
  2. 1 tube (Transparent or dark) or 96-well and 384-well plate
  3. Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.