Oligo Modification & Labeling

Synbio Technologies provides oligo synthesis and modifications. Our specialized services include oligonucleotides modified with special bases, dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, digoxin, etc. Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’ or designated sites specified by the customer. This includes FAM, HEX, ROX, BHQ, Texas Red, and much more. The resulting oligos can be used in a variety of applications, including PCR, cloning, sequence verification, and gene detection.

Competitive Advantages

  • Comprehensive Modifications and Labeling: Large-scale sequencing verifying the average mutation rate to be lower than 1/1000
  • Strict Quality Control: Stringent ISO9001 & ISO13485 Certified Processing for oligo synthesis, meticulously monitored and advanced traceability
  • Multiple Purification Options: Include desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
  • Technical Support: Professional teams experienced in oligo synthesis and various modifications offer support throughout the production progress

Services Specifications

Synbio Technologies provides a series of general modifications with the high purity and competitive prices. We accomplish this by focusing on the requests of modification with special chemical classifications. Browse general modifications listed below:

ClassificationModifications
Purification
Modified Bases
dU
HPLC/PAGE
dI
5-Methyl dC
DBCO
5’ DBCO
3’ DBCO
dT-DBCO
AMCA
5’ AMCA
3’ AMCA
dT-AMCA
Phosphorothioates
SPO3
Amino Linkers
5’Amino C6
dT-Amino C6
3’Amino C7
5’Amino C12
Biotin
5’Biotin
3’Biotin
dT-Biotin
Digoxigenin
5’Digoxigenin
3’Digoxigenin
dT-Digoxigenin
Phosphorylation
5’ PO4
3’ PO4
Hydrosulphonyl
5’ HS-C6
3’ HS-C3
Locked Nuleic Acid
LNA (A/G/C/U)
Fluoro Bases
2’-F-2’-dA
2’-F-2’-dC
2’-F-2’-dG
2’-F-2’-dU
Spacer
dSpacer
Spacer C3
Spacer C9
Spacer C18

*The above is our suggested purification method. If you need other purification methods, please contact us for a quote.

Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ and Texas Red, etc.

Dye/Quenchers3′-5′-Designated SitesPurification
FAM
HPLC/PAGE
TET
JOE
HEX
SIMA
TAMRA
ROX
Cy5
Cy3
Quasar670
Quasar570
DyLight547
DyLight647
Texas Red
BHQ-1
BHQ-2
BHQ-3
Eclipse
DABCYL

*The above is our suggested purification method. If you need other purification methods, please contact us for a quote.

Despite of single fluorescent group, Synbio Technologies also offers dual/multiple labeling services including TAMRA, BHQ, DABYCL, ELCISPE, etc. The most common Dyes and Quenchers are listed below:

DyesQuenchersPurification
5’6-FAM
3’TAMRA
HPLC/PAGE
3’BHQ-1
3’Eclipse
3’DABCYL
5’TET
3’TAMRA
3’BHQ-2
3’DABCYL
5’HEX
3’TAMRA
3’BHQ-1
3’BHQ-2
3’DABCYL
5’TAMRA
3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
5’ROX
3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
5’CY5
3’BHQ-1
3’BHQ-2
3’BHQ-3
3’DABCYL
5’CY3
3’BHQ-2
5’JOE
3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
3’TAMRA
5’Texas Red
3’BHQ-2

*The above is our suggested purification method. If you need other purification methods, please contact us for a quote.

Standard Deliverables

  1. Lyophilized dry DNA oligo powder
  2. 1 tube (Transparent or dark) or 96-well and 384-well plate
  3. Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.