Protein Expression Service | Synbio Technologies

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Protein Expression

In the rapidly advancing field of biological drug discovery and recombinant protein expression technology, there is growing demand for fast, cost effective, and functional protein molecules.

At Synbio Technologies, we have developed five major protein expression platforms: bacterial, yeast, insect, mammalian and cell-free. With our extensive experience, we supply thousands of recombinant proteins and antibodies worldwide each year. Our customization services include the systematic selection of expression vectors, hosts, and fusion tags based on protein characteristics and client reuirements. Additionally, our fermenter production services, ranging from 10L to 500L, cater to production needs from milligrams to grams, providing customers with significant savings in both time and cost.

	Cell-Free	Prokaryotic	Yeast	Insect	Mammalian
Expression Speed	Hours	2-3 Weeks	4-7 Weeks	5-9 Weeks	3-4 Weeks
Cost	Medium	Low	Medium	Medium	High
Expression Yield	++	++++	+++	+++	++
Post-Translational Modifications	－ (Poor)	－－－－ (None)	++ ((Limited))	+++ (Good)	++++ (Full)
Protein Quality (Folding/Activity)	++ (Optimizable)	﹣﹣﹣﹣ (Pool)	++ (Good)	+++ (Good)	++++ (Best)
Suitable Applications	Complex proteins; membrane proteins; toxic Proteins; small-scale	Non-modified Proteins; high-throughput; small molecules	Some glycoproteins; industrial scale	Complex proteins; membrane proteins	Drug development; glycoproteins

How can AI-designed proteins be turned into functional proteins?

We combines the power of AI design and synthetic biology to provide comprehensive support from protein sequence optimization to expression to functional validation, facilitating downstream applied research on protein prediction, bringing AI-designed proteins to life.

AI-Designed Proteins: Cell or Cell-Free?

Although AI accelerates protein design, testing these designs in living cells remains slow and problematic. Cell-free protein synthesis offers a faster, more flexible testing platform, enabling a new paradigm for protein engineering.

Highlights of Protein Expression Service

>95% Success Rate
Free Codon Optimization
Highly Customizable, Flexible Expression Systems
Numerous Self-developed High-efficiency Expression Vectors
Scalable Production from 10L-500L
Directed Evolution to High Performing Proteins

Service Details

FAQs

What protein expression system to choose？

Firstly, it mainly depends on the customer's choice or downstream applications.

If customers expect proteins to have post-translational modifications, activity, etc., they can choose from three eukaryotic systems (Among the three eukaryotic systems, we prefer to the mammalian system).

If customers have a high demand for protein, they can choose expression system in E.coli, which has a high expression level and relatively low cost.

If the customer does antigen testing, they can choose expression system in E.coli.

If the customer provides sequence information, we will search for relevant products and literature references. The final system still needs to be chosen by the customer.

Can we guarantee protein activity？

No, recombinant proteins cannot guarantee activity, and activity needs to be tested by the customer.

How to improve protein expression?

You can try from the following aspects.

If there are rare codons, you can try to perform codon optimization again (i.e. requiring gene synthesis).

If the protein molecular weight is too large or too small, it can be attempted to truncate expression or fuse large tag expression (i.e. plasmid reconstruction is required).

If it is a toxic protein, you can try the pLysS strain.

Replace the expression system (i.e. require re gene synthesis).

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