General Introduction of shRNA Library Customization
Short hairpin RNA (shRNA) is an RNA molecule delivered via vectors and stably expressed in cells, which can be processed into small interfering RNA (siRNA) to guide the RNA-induced silencing complex (RISC) in mediating the degradation of target messenger RNA (mRNA), thereby achieving specific gene knockdown. A single shRNA experiment can only investigate the function of one specific gene. However, most biological phenomena—such as cell proliferation, death, differentiation, carcinogenesis, and drug response—are not determined by a single gene but are co-regulated by complex gene networks.
The fundamental rationale for establishing shRNA libraries lies in the complexity of biological questions, with their core role being the transformation of RNAi technology into a powerful large-scale discovery tool. Using its proprietary Syno® synthesis technology platform, Synbio Technologies enables rapid and efficient construction of high-quality shRNA libraries, meeting diverse clients' customized needs and providing an efficient and cost-effective solution for large-scale loss-of-function screening.
Highlights
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- One-stop service：From shRNA design, library construction to lentivirus packaging
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- High coverage and high uniformity : library coverage > 90 %, and the abundance of each sequence is evenly distributed.
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- Rapid Viral Packaging: Provides cost-effective lentiviral packaging with short turnaround times.
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- NGS sequencing verification
Service Details
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Service Steps
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Service Details
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Turnaround Time
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shRNA Design
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shRNA sequences are designed for specific gene
3-6 shRNAs for each target gene
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2-3 days
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Oligo Pool Synthesis
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All designed shRNAs and negative controls will be
synthesized simultaneously
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3-4 weeks
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shRNA Library Construction
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Cloning shRNAs pool onto the target vector
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4 weeks
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Plasmid Purification
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Transfection-grade plasmid preparation to meet the
shipping need
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1 week
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Library Quality
NGS
Verification(Optional)
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100x greater library coverage ,
>65% library accuracy rate
3.>90% the coverage rate
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2-3 weeks
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Virus Packaging
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Virus liquid >108TU/mL
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2-3 weeks
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Workflow
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Related Services
DNA Synthesis
Vector Selection
Molecular Biology
Oligo Synthesis
RNA Synthesis
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Oligo Pools
Virus Packaging
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DNA Data Storage
Standard Oligo
Standard Genome KO Libraries
Standard Genome KO Plasmid
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